香蕉纤维脱胶茵的筛选及其产酶条件的初步研究

摘要

Banana fiber is a new raw material for textile industry. Shortage of retting ways and process limits the exploitation and application of banana fiber. This paper deals with the retting of banana fibers with microbial methods. Screened with a method of transparent zones, an actinomyces strain 51181 decomposing banana hemicellulose was isolated from the rhizosphere soil. The strain could degrade about 38％ of the gum of banana fiber by retting experiment in the lab, The optimal conditions for hemicellulase production by the strain 51181 were determined. The activity of hemicellulase reached to 48.63 U/mL under the optimum conditions. It showed that the strain 51181 had good applying prospects in microbial retting and enzymatic retting of banana fiber.%香蕉纤维是纺织行业的一种新型纤维原料,其脱胶方法和工艺研究的缺乏制约着相关的开发与利用.本文就香蕉纤维的微生物脱胶进行了初步研究,采用透明圈法从土壤中筛选到一株具有降解香蕉半纤维素能力的放线菌菌株51181,利用它脱胶可去除香蕉纤维38%的胶质;对菌株51181产半纤维素酶的条件优化后,该菌株所产的半纤维素酶活达48.63 U/mL,表明该菌株在香蕉纤维微生物脱胶及酶法脱胶方面具有一定的应用前景.