为了解多花水仙在开花过程中色素物质含量的变化,以多花水仙‘金盏银台’和‘黄花水仙2号’4个花期副冠和花被为材料,采用高效液相色谱法(HPLC)测定2种类胡萝卜素物质和11种类黄酮物质的含量.结果表明:多花水仙在花蕾期已经有大量色素物质积累；黄花水仙2号主要色素物质是芦丁和叶黄素,金盏银台的主要色素物质是芦丁、柚皮苷、阿魏酸和叶黄素;花期2个品种副冠和花被中芦丁、柚皮苷和阿魏酸的含量变化趋势基本一致,均表现为花蕾期含量最高,始花期、盛花期有所降低,衰败期又升高的趋势,而叶黄素含量变化不尽相同;经t测验分析,花期不同品种同一部位中芦丁、柚皮苷、阿魏酸和叶黄素的含量差异显著,同一品种不同部位中叶黄素和阿魏酸的含量差异显著,芦丁、柚皮苷的含量差异不显著.%In order to understand the changes of pigment content in Narcissus Tazetta flower at flowering phase, the contents of carotenoids and flavonoids in perianth and corona of JINZHANYINTAI and YELLOWFLOWER II at flowering phase were tested by high performance liquid chromatography (HPLC). The results showed that there were a large number of various pigmentations accumulated in perianth and corona of Narcissus Tazetta, and rutin and phytoxanthin were the main pigmentations in YELLOWFLOWER II flowers, while rutin, naringin, ferulic acid and phytoxanthin were the main pigmentations in JINZHANYINTAI flowers. The dynamic trends of rutin, naringin and ferulic acid contents in the two varieties above were consistent at flowering phase: the contents in perianth and corona were the highest at flower budding period, were decreased somewhat at early flowering stage and full-bloom stage, and further increased at decline stage. But the phytoxanthin content change was not the same. Analysis of t -test indicated that there was a significant difference in the contents of rutin, naringin, ferulic acid and phytoxanthin between the varieties, respectively, and there was a significant difference in phytoxanthin and ferulic acid contents between perianth and corona in the same variety, respectively, but not significant difference for rutin and naringin contents, respectively.
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