多蕊蛇菰不同提取物及其抑菌活性

摘要

The crude extracts of Balaophora polyandra were extracted by methanol. The thin layer chromatography-bioautography assay and radial growth assay were used for inhibitory activities against different bacteria and Colletotrichum gloeosporioides. The results showed that the ethyl acetate layer extracts showed the strongest inhibitory activity on the bacteria, followed by n-butanol layer crude, and the petroleum ether extract showed the weakest activity; All extracts showed the strongest inhibitory activity on Ralstonia solanacearum. The ethyl acetate crude extracts also showed the best inhibitory activity against Colletotrichum gloeosporioides. and the EC50 value was 0.89 mg/mL. The EC50 value of n-butanol crude extract was 6.52 mg/mL. The petroleum ether extracts didn't show any anti-fungus activity under the test concentration.%采用甲醇冷浸提取法提取多蕊蛇菰次生代谢产物,并通过薄层层析-生物自显影法和含毒平板法分别测定了次生代谢产物对不同供试细菌和降香黄檀炭疽病菌的抑制活性.结果表明,多蕊蛇菰乙酸乙酯层提取物对供试细菌的抑制活性最强,其次是正丁醇层,石油醚层提取物的活性最弱;所有提取物对桉树青枯病菌均表现出最强的抑制活性.多蕊蛇菰乙酸乙酯层提取物对降香黄檀炭疽病菌的抑制活性强,EC50值为0.89 mg/mL,正丁醇层次之,EC50值为6.52 mg/mL,石油醚层提取物在供试浓度下未表现出抑制活性.