热休克蛋白70封闭后对星形胶质细胞反应性增生的影响

摘要

Objective To study the changes of reactive astrocytosis after heat shock protein 70 (HSPT0) was blocked by anti-HSP70 antibody. Methods We established cell model of scratch inju-ries by in situ culture and prurification of rat astrocytcs. Anti-HSP70 antibody was added into the nutrient medium at once after injury for intervention (intervention group). Then, immunocytochemical staining of glial fibrillary acidic protein (GFAP) was done at different time points in control group and intervention group to observe astrocytosis and morphologic changes, mRNA expression of GFAP was observed by means of reverse transcriptase-polymerase chain reaction (RT-PCR). Results Compared with the con-trol group, average cell area, average dentritic length and number of dentrities of astrocytes were signifi-cantly reduced in the intervention group(P < 0.05 or P < 0.01), with down-regulated mRNA expression of GFAP (P < 0.05). Conclusion HSP70 plays a facilitative role in reactive astrocytosis after injury of astrocytes. Reactive astrocytosis can be controlled to some extent by blocking HSP70 with anti-HSP70 antibody.%目的 探讨用抗体封闭热休克蛋白70(heat shock protein 70,HSP70)后对星形胶质细胞反应性增生的影响. 方法 原代培养并纯化星形胶质细胞,采用划痕损伤法构建细胞损伤模型,通过培养液内加入HSP70抗体来进行干预.在不同时间点对损伤对照组和损伤干预组进行胶质纤维酸性蛋白(GFAP)免疫细胞化学染色,观察细胞增生情况及形态学变化,并用RT-PCR检测GFAP mRNA的表达. 结果 与对照组相比,干预组细胞面积、突起数目及突起长度均减小(P<0.05或0.01),GFAP mRNA的表达也明显降低(P<0.05). 结论 星形胶质细胞损伤后,HSP70有促进星形胶质细胞反应性增生的作用.用抗体封闭HSP70后,能够一定程度上抑制星形胶质细胞的反应性增生.