局部转染Ngn2基因对大鼠实验性急性脊髓损伤运动功能影响的研究

摘要

Objective:To investigate the effect of local Ngn 2 plasmid transfer on motor function in rats with acute spinal cord injury motor function.Methods:36 four-month-old SPF SD rats were randomly divided into three groups ,namely Group A (sham group), Group B (experimental group), Group C (control group),n =12.Sham group was only bite T8-T10 spinous process and lamina with-out damage to the spinal cord , the experimental group and the control group made the T 8 acute spinal cord injury model by using modified Allen's method.Postoperatively, 5μg Ngn2 plasmid for Group B and 5μg vector plasmid for Group C were continuously injected into the spinal cord segments .1,2,3,4 weeks after surgery to detect motor function in rats using the BBB motor function scoring system , 1 week after surgery to test the expression of Ngn 2 gene of spinal cord injury tissue by RT -PCR and 4 weeks after surgery detect spinal pathology by HE staining or the number of Nestin +cells by SP staining .Results:Postoperative BBB score the experimental group at each time point was significantly better than the control group .It was statistically significant (P <0.05).There was the expression of Ngn2 gene in spinal cord injury tissue of the experimental group , no expression in the control group .The experimental group had Nestin +cells (14. 25 ±2.37) ones per a single vision, while the control group Nestin +cells (2.54 ±1.14) ones, the difference was statistically sig-nificant (P <0.05).Conclusion:Ngn2 gene transferred by local injection has significant effect on the motor function recovery in rats with acute spinal cord injury , which may induce proliferation of the endogenous neural stem cells in the spinal cord .%目的：研究局部转染Ngn2基因对急性脊髓损伤大鼠运动功能的影响。方法：将36只4月龄SPF级SD大鼠，随机分成3组，分别为A组（假手术组）、B组（实验组）、C组（对照组），每组12只。假手术组只咬除T8～T10棘突及椎板、不损伤脊髓，实验组和对照组采用改良Allen's打击法制造T8急性脊髓损伤模型，术后分别向B、C 2组损伤脊髓节段持续注射Ngn2质粒和空载体质粒各5μg。于术后1、2、3、4周采用BBB运动功能评分系统检测大鼠运动功能，术后1周应用RT-PCR检测损伤节段脊髓组织Ngn2基因表达，术后4周HE染色脊髓病理学检测及SP染色检测Nestin＋细胞数。结果：术后各时间点实验组BBB评分明显优于对照组，有统计学意义（P＜0．05）；实验组大鼠脊髓组织中Ngn2mRNA有表达，而对照组无表达；实验组单个视野Nestin＋细胞为（14．25±2．37）个，对照组单个视野Nestin＋细胞为（2．54±1．14）个，差异有统计学意义（ P＜0．05）。结论：Ngn2基因能够促进急性脊髓损伤大鼠运动功能恢复，可能与诱导脊髓内源性神经干细胞的增殖有关。