枳椇子对非酒精性脂肪肝大鼠血清TNF -α、IL -6、IL -8水平的影响

摘要

Objective: To observe the effects of hovenia didcis thunb on histological changes of liver and serum TNF - a, IL - 6, and IL - 8 levels in rats with non - alcoholic fatty liver, discuss the mechanism.Methods: SD rats were feeding high fat diet for 90 days to build non - alcoholic fatty liver models, different doses hovenia dulcis thumb liquid were given to intervene the formation of non - alcoholic fatty liver for 90 days at the same time. The histological changes of rats' liver were observed by light microscope, serum TNF - a, IL - 6,and IL - 8 levels wre determined by radioimmunoassay. Results: The degree of fatty degeneration and inflammation activity of hepatic tissue in model group was obvious, serum TNF - a, IL - 6, and IL - 8 levels of model rats were significantly higher than those of normal rats; the degree of inflammation activity of hepatic tissue in high and low dose hovenia dulcis thunb groups was obviously improved, serum TNF - a, IL - 6, and IL - 8 levels in the two dose hovenia dulcis thumb groups were significantly reduced.Conclusion: TNF - a, IL - 6, and IL - 8 were related to the occurrence of non - alcoholic fatty liver induced by high fat diet, decreasing serum TNF - a, IL - 6, and IL - 8 contents maybe one of the mechanism that hovenia dulcis thumb can effectively prevent non -alcoholic fatty liver.%目的:观察枳椇子对高脂饮食诱导的非酒精性脂肪肝(NAFLD)大鼠肝组织学变化和血清TNF-α、IL -6、IL -8水平的影响,探讨其防治NAFLD的作用机理.方法:以高脂饮食喂养SD大鼠90天建立非酒精性脂肪肝模型,同时以不同剂量的枳楔子连续干预90天,观察肝组织学的病理改变,放射免疫法测定血清TNF -α、IL -6、IL -8水平.结果:模型组大鼠肝组织脂肪变性、炎症活动度、血清中TNF -α、IL -6、IL -8水平较正常组明显升高；枳棋子高、低剂量组大鼠肝组织炎症程度、血清TNF -α、IL -6、IL -8含量明显降低.结论:TNF -α、IL -6、IL -8参与高脂饮食诱导的大鼠非酒精性脂肪肝的发生；降低血清TNF -α、IL -6、IL -8水平可能是枳椇子防治非酒精性脂肪肝进展的重要机制之一.