神经保护剂的鸡尾酒疗法对大鼠局灶性脑缺血的作用

摘要

AIM: To investigate whether cocktail theraphy combined with of neuropro-tectants may have more advantages over single agents in treating focal cerebralischemic cascade. METHODS: With the use of suture occlusin techique, theright middle cerebral artery in rats was occluded. Tirty minutes later, Fru-toss-1, 6-diphosphate(FDP) (50 mg/kg, n = 20), MK-801 (1 mg/kg, n = 20)and N-acetylcystein (NAC) ( 150 mg/kg, n = 20) were singly or combinantlyinfused intraperitoneally. At the same time the cocktail-treated group( n = 20)were infused the above agents combinationly and the control group( n =20)were infused normal saline intraperitoneally. 6 hours and 24 hours after focalcerebral ischemia the animals were weighted and neurologically assessed on5-point scale. The animals were killed, brains were stained 2, 3,5-triphenyltetrazolium chloride for assessment of the infarct volume and thenembedden onto slides with paraffin for morphological assessment and terminaltransferase dUTP nick ending labeling(TUNEL )were carried out for apoptosisand immunohistochemistry were carried out to investigate the changes inbcl-2. RESULTS: All Neuroprotectants decreased volume of infarction ( P ＜0. 05, F test) . While cocktail-treated group showed more distinct decreasethan other groups( P ＜ 0.05, F test). FDP-treated group did not decrease theapoptosis of the neurons and did not increase the bcl-2 expression aswell. MK-801-treated group, NAC-treated group and cocktail-treated groupsignificantly decreased the apoptosis of the neurons and increased the bcl-2expression ( P ＜ 0.05, F test) . With cocktail-treated group showing moredistinct effect ( P ＜ 0.05, F test) . CONCLUSION: Cocktail may be moreeffective than single neuroprotectant in this modle.