背景:文献报道人参皂甙Rg3具有抗肿瘤作用,但对白血病作用研究很少.目的:观察人参皂甙Rg3对白血病K562细胞的作用,并探讨其相关机制.方法:以白血病K562细胞为靶细胞,实验分为对照组和人参皂甙Rg3组,人参皂甙Rg3组分别添加10,20,40,80,100 mg/L Rg3.结果与结论:MTT检测结果显示,不同浓度人参皂甙Rg3组K562细胞生长抑制率均显著高于对照组(P < 0.05～0.01).NBT结果显示,人参皂甙Rg3组培养1,2,3 d K562细胞还原率均高于对照组(P < 0.01);人参皂甙Rg3组部分K562细胞体积变小,核仁消失,同时阳性细胞增多,细胞向成熟分化;流式细胞仪检测显示人参皂甙Rg3组培养2 d细胞周期G2期增高了3.84倍.提示人参皂甙Rg3在体外可抑制K562细胞增殖活性,其机制可能与人参皂甙Rg3将K562细胞周期阻滞在G2期,使细胞不能进行正常的有丝分裂,从而抑制细胞增殖有关.%BACKGROUND: Evidence exists that ginsenoside Rg3 can inhibit the growth of carcinoma cells. However, there are fewer studies describing ginsenoside Rg3 effects on leukemia.OBJECTIVE: To investigate the effects of ginsenoside Rg3 on proliferation of erythroleukemia cell line K562 and the underlying mechanism.METHODS: Erythroleukemia cell line K562 was used as target cell. The cells were divided into a control group and an Rg3 group. 10, 20, 40, 80, 100 mg/L Rg3 was added in the Rg3 group.RESULTS AND CONCLUSION: The MTT colorimetric assay indicated that K562 growth inhibition rate was significantly higher in the Rg3 groups than in the control group (P < 0.05-0.01). The nitroblue tetrazolium assay showed that after culture for 1,2, 3 days, the reducing power of K562 cells in the Rg3 group was significantly higher than in the control group (P < 0.01). In the Rg3 group, some K563 cell somas became small, nucleoli disappeared, nitroblue tetrazolium positive cells increased, and cells developed toward maturation. Flow cytometry detection showed that after 2 days of culture, the cell cycle at G2 phase in Rg3 group was 3.84 times higher than that in the control group. Ginsenoside Rg3 can inhibit the proliferation of erythroleukemia cell line K562 in vitro. The underlying mechanism may be related to a fact that ginsenoside Rg3 blocks K562 cell cycle at G2 stage, hinders normal mitosis and thereby inhibits cell proliferation.
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