背景:研究发现人源羊水干细胞可向心肌前体细胞分化.目的:观察不同浓度重组人促红细胞生成素对人源羊水干细胞向心肌前体细胞分化的影响.方法:用含0,1.0,5.0,10.0,20.0 U/mL 重组人促红细胞生成素的诱导培养基诱导鉴定后的人源羊水干细胞向心肌前体细胞分化,诱导24 h 后换为完全培养基继续培养.结果与结论:诱导分化14 d,倒置显微镜下可见细胞由长梭形逐渐变短增宽,相邻的细胞间有融合现象,似类肌管样结构,以5.0 U/mL 重组人促红细胞生成素的诱导分化率最高;RT-PCR 检测显示重组人促红细胞生成素可促进细胞Nkx-2.5 和GATA-4 mRNA 的表达,以5.0 U/mL 重组人促红细胞生成素的作用最强.说明外源性重组人促红细胞生成素能剂量依赖性促进人源羊水干细胞向心肌前体细胞分化.%BACKGROUND: Studies have shown that amniotic fluid stem cells of pregnancy women can differentiate into myocardial precursor cells. OBJECTIVE: To study the effect of recombinant human erythropoietin (rhEPO) at different concentrations on differentiation of myocardial precusor cells from amniot I c fluid stem cell s of pregnancy women. METHODS: The differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women was induced by adding conditional culture medium containing 0, 1.0, 5.0, 10.0, 20.0 U/mL rhEPO. After 24 hours, conditional culture medium was replaced by complete medium. RESULTS AND CONCLUSION: During 14 days of induced differentiation, under the inverted microscope, cells gradually shortened and widened from original shuttle appearance. Adjacent cells fused together, similar to myotube- like structure. The differentiation rate of myocardial precursor cells was highest after 5.0 U/mL rhEPO addition. RT-PCR results showed that rhEPO particularly at the concentration of 5.0 U/mL could promote Nkx-2.5 and GATA-4 mRNA expression. These findings suggest that exogenous rhEPO can promote the differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women dose-dependently.
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