人鼻咽癌CD133+干细胞的生物学特性及意义人鼻咽癌CD133+干细胞的生物学特性及意义

摘要

背景:有研究表明肿瘤细胞株中存在肿瘤干细胞,是肿瘤复发、转移的根源,但人鼻咽癌细胞株CNE-2中肿瘤干细胞的表达及生物学特性至今少有报道.目的:观察人鼻咽癌CD133+干细胞生物学特性及意义.方法:采用流式细胞仪检测人鼻咽癌细胞株CNE-2中CD133的表达情况.免疫磁珠分选技术获得人鼻咽癌CD133+干细胞,分别采用无血清培养法、CCK-8法、平板克隆形成试验及裸鼠体内成瘤实验检测CD133+干细胞的体外增殖及体内成瘤能力,并将其与CD133-及未分选鼻咽癌细胞进行比较,以了解CD133+干细胞的生物学特性.结果与结论:免疫磁珠富集的CD133+细胞在无血清培养基中呈悬浮生长,并可以形成肿瘤干细胞球.CD133+细胞与CD133-细胞比较具有较高的克隆形成能力(P < 0.01);CD133+细胞在裸鼠体内的成瘤率高于CD133-细胞(P < 0.05).结果证实,鼻咽癌CD133+干细胞能在体外分离培养,形成干细胞球,增殖能力强,在裸鼠体内具有极强的成瘤能力.%BACKGROUND: Recent studies shows that cancer stem cells exist in cancer cell lines, and may be responsible for recurrence and metastasis of tumor. There are few reports on the biological characteristics of CD133+ cancer stem cells in human nasopharyngeal carcinoma.OBJECTIVE: To explore the biological characteristics and significance of CD133+ cancer stem cells in human nasopharyngeal carcinoma.METHODS: Expression of theCD133+ cells were detected by flow cytometry, and purified by magnetic cell sorting from human nasopharyngeal carcinoma cell line CNE-2. In vitro proliferation and in vivo tumorigenic capacity of the CD133+ stem cells were detected by serum-free culture, CCK-8, plate colony formation test and tumorigenicity experiment in nude mice, and which statistically compared with CD133- and control CNE-2 cells to understand the biological characteristics of the CD133+ stem cells.RESULTS AND CONCLUSION: CD133+ cells maintained a sphere-like growth status in serum-free medium in vitro, and could form tumor spheres. Compared with the CD133- cells, the CD133+ cells had higher colony-formation ability (P< 0.01). In vivo tumorigenesis ability of the CD133+ cells in the nude mice was higher than that of the CD33- cells (P = 0.05). The results confirmed that CD133+ cells can successfully separate and culture in vitro, and can generate tumor spheres and have high tumorigenesis ability in the nude mice.