脂肪间充质干细胞移植后肝硬化大鼠血生化指标的变化

摘要

背景：干细胞移植是晚期肝病比较有前景的治疗方法，脂肪间充质干细胞是继骨髓间充质干细胞后的又一研究热点。　　目的：观察脂肪间充质干细胞移植后对肝硬化大鼠血生化指标的影响作用。　　方法：将60只大鼠随机分为正常对照组、模型组及脂肪间充质干细胞组，各20只。模型组及脂肪间充质干细胞组采用四氯化碳灌胃诱导建立肝硬化大鼠模型。建模成功后1周，脂肪间充质干细胞组腹腔注射脂肪间充质干细胞悬液，正常对照组及模型组注射生理盐水。　　结果与结论：与对照组相比，模型组大鼠肝组织中丙氨酸氨基转移酶、门冬氨酸氨基转移酶、总胆红素、总蛋白含量及血清丙二醛含量显著升高，血清白蛋白、血清蛋白含量与球蛋白比值及肝组织中谷胱甘肽过氧化物酶及环磷酸鸟苷含量明显下降，且吲哚氰绿15 min滞留率及肝纤维化程度明显上升，而与模型组相比，脂肪间充质干细胞组相比丙氨酸氨基转移酶、门冬氨酸氨基转移酶、总胆红素、总蛋白含量及血清丙二醛含量显著下降，血清白蛋白、血清蛋白含量与球蛋白比值肝组织中明显升高，且吲哚氰绿15 min滞留率及肝纤维化程度明显减轻，同时脂肪间充质干细胞组大鼠肝脏组织中可见CM-Dil标记的阳性脂肪间充质干细胞。提示脂肪间充质干细胞能够影响大鼠肝硬化的血生化指标水平，脂肪间充质干细胞移植后可以减轻大鼠肝硬化程度。%BACKGROUND:Stem cel transplantation is a promising treatment of advanced liver disease, and adipose-derived mesenchymal stem cel s have become another kind of popular cel s fol owing bone marrow mesenchymal stem cel s. OBJECTIVE:To investigate the influence of adipose-derived mesenchymal stem cel transplantation on blood biochemical indices of liver cirrhosis rats. METHODS:Sixty rats were equal y randomized into normal control, model and cel transplantation groups. Model rats of liver cirrhosis were made in the latter two groups through intragastric administration of carbon tetrachloride. One week after successful modeling, rats were given intraperitoneal injection of adipose-derived mesenchymal stem cel suspension in the cel transplantation group, and given normal saline in the other two groups. RESULTS AND CONCLUSION:Compared with the normal control group, the model group showed a significant increase in the levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, total protein in liver tissues, serum level of malondialdehyde, 15-minute indocyanine green retention rate and degree of hepatic fibrosis, and a significant decrease in serum albumin level, serum albumin/globulin, levels of glutathione peroxidase and cyclic guanosine monophosphate in liver tissues. On the contrary, these indicators were al improved in the cel transplantation group compared with the model group. Moreover, CM-Dil-positive cel s were visible in the liver tissue of rats undergoing adipose-derived mesenchymal stem cel transplantation. Al these findings indicate that adipose-derived mesenchymal stem cel transplantation can reduce liver cirrhosis in rats by acting on blood biochemistry levels.