背景:研究发现,骨骼肌中的 FAT/CD36通常是在运动的刺激作用下诱导骨骼肌收缩向细胞膜外转运,通过转运增加了细胞膜的FAT/CD36含量,从而增加了脂肪酸进入骨骼肌细胞的数量.目的:观察运动前补充甘草黄酮大鼠股四头肌病理改变和脂肪酸转移酶FAT/CD36的影响.方法:SD 雄性大鼠30只,随机分为安静对照组、运动对照组、运动+甘草黄酮组.运动+甘草黄酮组按10.0 mL/(kg ? d)进行灌服给药,对照组给等量生理盐水;并且运动2组均进行为期6周的训练,速度为19.3 m/min, 跑台坡度为5°.苏木精-伊红染色观察股四头肌的病理学变化;免疫组织化学法和Western Blot法测定FAT/CD36的表达.结果与结论:①光镜下观察运动对照组大鼠股四头肌纤维有肿胀、破裂和坏死的现象,运动+甘草黄酮组股四头肌纤维细胞排列较紧密,肌纤维边缘完整,与安静对照组比较无明显的差异;②免疫组织化学反应显示,运动对照组大鼠股四头肌 FAT/CD36表达高于安静对照组(P < 0.05),运动+甘草黄酮组大鼠股四头肌FAT/CD36表达略高于安静对照组,但低于运动对照组(P < 0.05);③Western Blot检测结果与免疫组织化学具有相同的趋势;④结果说明,运动诱导增加了脂肪酸转运能量,出现积累的效应;FAT/CD36通过甘草黄酮协同作用下增加了脂肪酸的运转效率,从而维持骨骼肌脂质中的能量代谢稳态在一定的水平上,防止脂质代谢紊乱.%BACKGROUND: Fatty acid translocase/CD36 (FAT/CD36) in skeletal muscle has been proved to induce skeletal muscle contraction transport to the outer membrane stimulated by movement, which increases FAT/CD36 content in cellular membrane, further leading to more fatty acids into skeletal muscle cells. OBJECTIVE: To observe the effects of Glycyrrhiza Flavone administration before exercise on the pathological changes of quadriceps and FAT/CD36 in rats. METHODS: Thirty male Sprague-Dawley rats were randomly divided into quiet, exercise and combination groups. The rats in the combination group were administrated with 10 mL/(kg?d) Glycyrrhiza Flavone, while the other two groups were given the same volume of normal saline. The rats in the exercise and combination groups were subjected to 6-week training, 19.3 m/minute at the treadmill slope of 5°. The pathological changes of quadriceps were observed by hematoxylin-eosin staining, and the expression level of FAT/CD36 was detected by immunohistochemistry and Western blot assay. RESULTS AND CONCLUSION: Under light microscope, swollen, ruptured and necrotic muscle fibers in the quadriceps were found in the excise group. In the combination group, muscle fibers tightly arranged in the quadriceps with complete edge, which had no significant difference compared with the quiet group. Immunohistochemistry showed that the expression level of FAT/CD36 in the exercise group was significantly higher than that in the quiet group (P < 0.05); the expression level of FAT/CD36 in the combination group was slightly higher than that in the quiet group, but significnatly lower than that in the exercise group (P < 0.05), all above findings were consistent with the western blot assay. Our results indicate that exercise can induce fatty acid transport and accumulation. Due to the synergistic action of Glycyrrhiza Flavonoids, FAT/CD36 increases the efficiency of fatty acid transport, thereby maintaining lipid metabolism balance in the skeletal muscle, which avoids lipid metabolic disorders.
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