半乳糖凝集素3及骨桥蛋白在非小细胞肺癌亚临床转移中的意义

摘要

目的 研究半乳糖凝集素-3(Gal-3)和骨桥蛋白(OPN)在非小细胞肺癌(NSCLC)中的表达及其与亚临床转移的关系.方法 选取2006年1月至2007年10月接受外科治疗的NSCLC患者46例,男性28例,女性18例,年龄33～77岁.应用RT-PCR及ELISA分别检测患者肺癌组织和血 浆中Gal-3和OPN的表达,并结合术后随访,对肿瘤转移组和非转移组进行比较.结果 46例NSCLC患者中,12例术后出现肿瘤转移.转移组血浆Gal-3水平为(18.8±7.9)μg/L,高于非转移组的(9.2±5.6)μg/L.转移组血浆OPN为(153.5±63.5)μg/L,高于非转移组的(89.2±24.0)μg/L.转移组癌组织中Gal-3 mRNA含量为0.73±0.23,OPN mRNA含量为0.79±0.24,均高于非转移组(0.07±0.17和0.17±0.25).结论 Gal-3和OPN是NSCLC的一种重要负性预后因素.血浆中Gal-3和OPN的高表达有可能成为判断NSCLC亚临床转移的有效指标之一.%Objective To study the relationship between expression of galectin-3 (Gal-3) and esteopontin (OPN) in occult metastasis in non-small cell lung cancer. Methods Forty-six patients of non-small cell lung cancer (NSCLC) from January 2006 to October 2007 were selected. There were 28 males and 18 females, aged from 33 to 77 years old- The levels of lung tissues Gal-3 and OPN were detected by RT-PCR, and the levels of blood plasma's were measured by ELISA. Results There were 12 patients who had metastasized. In un-metastasis group the Gal-3 and OPN mRNA expression levels were significantly lower than that in metastasis group: mean value were 0. 07±0. 17 and 0. 17 ±0. 25 in un-metastssis group, while 0. 73±0. 23 and 0. 79±0. 24 in metastasis group. Blood plasm levels of Gal-3 ( 18. 8±7. 9)μg/L and OPN (153.5±63. 5 ) μg/L in NSCLC which were detected from metastasis group were higher than un-metastasisgroupof(9.2±5.6)μg/Land (89.2±24.0)μ g/L. Conclusions High serum levels of Gal-3 and OPN and high expression of Gal-3 and OPN mRNA in NSCLC are closely related to the occurrence and metastasis of NSCLC. They may be indexes of evaluating the occult metastasis in NSCLC.