拔牙后大鼠三叉神经节钠通道蛋白Nav1.8及Nav1.9 mRNA的表达变化

摘要

Objective To observe the expression and function of extraction.Methods Real-time reverse transcription PCR paralleled with vitro.established CRNA standard Curves was applied to measure the expression of Nav1.8,Nav1.9 at 30 min,2 h,1 d,3 d and 6 d respectively after extraction of rat right mandibular molars.The right mandibular molars were used as control.Results Both Nav1.8 and Nav1.9mRNA in fight trigeminal ganglion showed little change after 30 min.and increased slowly after 2 h.Nay1.8.Nav1.9 mRNA expressions increased by 27%and 24.5%respectively compared to the left trigeminal ganglion after 3 d,reaching the highest level(P＜0.05),and then the expressions began decreasing from 6 d. Conclusions The pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Nav1.8 and Nav1.9 mRNA.indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.%目的 观察三叉神经节神经元胞体中钠通道蛋白Nav1.8、Nav1.9 mRNA在大鼠拔牙后的表达及作用,探讨拔牙术后外周组织疼痛的机制.方法 Wister成年鼠30只,分为5组,每组6只,拔除大鼠右下磨牙,取同侧与对侧自体对照.采用体外快速构建cRNA标准曲线实时(realtime,RT)反转录聚合酶链反应(PCR)法,分别检测拔牙后30 min、2 h、1 d、3 d、6 d时间点Nav1.8、Nav1.9mRNA的表达.结果 Nav1.8、Nav1.9 mRNA在拔牙后30 min无明显变化,2 h开始缓慢上调,至第3天时表达明显上调.在第3天Nav1.8、Nav1.9mRNA与对照组相比,分别上调27.0%、24.5%(P＜0.05),第6天表达开始下降.结论 拔牙后疼痛产牛与钠通道蛋白Nav1.8、Nav1.9mRNA表达上调有关,提示钠通道蛋白参与拔牙后外周组织疼痛的调节.