口腔扁平苔藓患者外周血微小RNA-155和146a的表达

摘要

Objective To investigate the expression and clinical significance of miRNA-155 and miRNA-146a in peripheral blood mononuclear cells(PBMC) and plasma of oral lichen planus(OLP) patients.Methods Twenty-five female and seven male OLP patients(OLP group) aged 25 to 54 years were selected from January 2012 to May 2013.The diagnosis was confirmed by pathology and the lesions were divided into two non-erosive OLP group(18 cases) and erosive OLP group(14 cases).Twenty healthy sex and age matched volunteers served as control,miRNA-155 and miRNA-146a expressions in PBMC and plasma were examined by real-time PCR.The difference between OLP group and control group was statistically analyzed.Results The expressions of PBMC and plasma miRNA-155 were higher in OLP patients than those in the healthy control (median,0.07 vs 0.03,P＜0.05; 5.84 vs 1.32,P＜0.01).The median expression level of miRNA-146a in PBMC and plasma of OLP patients and healthy controls were (1.26 vs 0.58,P＜0.05) and (412.60 vs 238.42,P＜0.01).The plasma miRNA-155 and miRNA-146a expressions were significantly higher in erosive OLP group than those in non-erosive OLP group.There were no significant differences in the expression of PBMC miRNA-155 and miRNA-146a between the two groups.Conclusions The expressions of PBMC and plasma miRNA-155 and miRNA-146a are higher in OLP patients.The expressions of plasma miRNA-155 and miRNA-146a are associated with OLP severity.The over expression of miRNA-155 and miRNA-146a in OLP may play a role in the pathogenesis of OLP.%目的 探讨口腔扁平苔藓(oral lichen planus,OLP)患者外周血单个核细胞(peripheralbloodmononuclearcell,PBMC)和血浆中微小RNA (micro RNA,miRNA) 155 (miRNA-155)及miRNA-146a的表达,阐明其在OLP发病中的作用及意义.方法 收集2012年1月至2013年5月南昌大学附属口腔医院口腔内科收治的OLP患者32例(OLP组),其中女性25例,男性7例,年龄25～54岁.所有OLP患者均为初诊且经病理学确诊,按照病损基部黏膜状况又分为非糜烂型(18例)和糜烂型(14例)OLP.选择20名性别、年龄匹配的健康志愿者作为健康对照组.采用实时荧光定量PCR技术检测两组受试者PBMC及血浆中miRNA-155、miRNA-146a的表达水平并进行统计学分析.结果 OLP组PBMC和血浆中miRNA-155的表达水平(中位数分别为0.07和5.84)均显著高于健康对照组(中位数分别为0.03和1.32),差异均有统计学意义(P＜0.05);OLP患者PBMC和血浆中miRNA-146a的表达水平(中位数分别为1.26和412.60)均显著高于健康对照组(中位数分别为0.58和238.42),差异均有统计学意义(P＜0.05).糜烂型OLP组血浆中miRNA-155和miRNA-146a的表达均显著高于非糜烂型OLP组,差异均有统计学意义(P＜0.05);PBMC中miRNA-155和miRNA-146a的表达与非糜烂型OLP组比较,差异均无统计学意义(P＞0.05).结论 OLP患者PBMC和血浆中miRNA-155、miRNA-146a的表达水平显著升高,且血浆中miRNA-155和miRNA-146a的表达变化与OLP病损严重程度有关,提示miRNA-155和miRNA-146a在OLP的发病中起一定作用.