基于干细胞的牙功能组织模块用于牙及其支持组织再生

摘要

Stem cell based generation of functional tissue modules is becoming a hotspot in the field of tissue regeneration. These functional tissue modules, which can be prepared through a variety of ways, including self-organization of stem cells, cell sheets, electrospinning and bio-printing, can serve as the basic unit and be assembled to form larger tissues and organs. This new technology can be a break-through to address current issues in tissue regeneration and further promote the translational use of regenerated tissues/organs. Construction of functional tooth modules is still in its early stage, where the stem cell source, preparing method and strategy of translational use are not clearly defined yet. How to construct the functional tissue module through the existing technology still needs further study. Here, we would like to share our current understanding and thoughts on constructing strategies and potential clinical application of functional tooth modules including dental pulp, periodontal tissue and tooth root, and hope it could shed new lights on the regeneration of tooth.%基于干细胞的模块化功能组织构建已成为组织再生领域的研究新热点,利用细胞自组装规律、细胞膜片技术、静电纺丝技术、三维生物打印技术等构建功能模块,再通过类似"搭积木"的方式将模块组织进行拼装,最终实现组织或器官的再生.这一技术有望突破现有组织工程技术的瓶颈问题,实现功能性组织器官的再生及临床转化应用.牙功能组织模块的构建还处于起步阶段,其干细胞来源、构建方法 、转化应用方案等尚不明确,如何通过现有技术构建牙功能组织模块仍需进一步研究.本文分别以牙功能组织模块中的牙髓、牙周及牙根功能组织模块为例介绍这3种牙功能模块的构建思路、策略及应用前景,为牙再生研究提供新的研究思路.