Objective To observe the effect of administrating major bupleurum decoction (MBD) in combination with exercise on the inflammatory cytokines of liver in rats with obstructive jaundice. Methods Forty SD rats were randomly divided into control group (CG, n=10) , obstructive jaundice group (OJ, n = 10) , MBD group (MBD, n = 10) , and MBD + exercise group (MBD + E, n = 10) . The obstructive jaundice model was established by bile duct hang. MBD group and MBD + exercise group were orally administered with 2ml MBD once a day for 12 weeks. MBD + E group ran ona treadmill with 5% slope at the speed of 16.8m/rain for 60min per day, 5 times a week, for a total 12 weeks. Liver samples of all rats were collected after 12 weeks. Pathological changes were observed by microscope; Expression of TLR4 and MyD88 proteins were detected with SABC; TNF-a, IL-6, and IL-10 mRNA expression were determined with RT-PCR; ALT, AST and TBIL were also measured. Results Liver cable arranged normally in CG group. Fibrosis, degeneration and necrosis in liver cell appeared in OJ group. Disorder of liver cable structure and light necrosis were seen and ALT, AST and TBIL reduced in MBD group (P<0.05) . Insignificant necrosis, significant reduction in ALT, AST and TBIL and basically normal lobular architecture were observed in MBD + E group as compared with those in OJ group (P <0.01) . Expression of TLR4 and MyD88 were significantly reduced in MBD and MBD + E groups as compared with those in OJ group. The expression of inflammatory mediators such as TNF-a and IL-6 in MBD + E group was lower than OJ group (P < 0.01) , while IL-10 level was significantly higher than that of OJ group (P < 0.01) . Conclusion The results in this study indicated that applying major bupleurum decoction in combination with moderate intensity aerobic exercise showed a better effect on adjusting the expression of liver inflammatory cytokines, and thus improved the rats' immunity and finally reduced the liver injury of rats with obstructive jaundice.%目的:观察中药大柴胡汤联合中等强度有氧运动对大鼠阻塞性黄疸致重症肝损伤肝脏炎症细胞因子的影响.方法:40只SD大鼠随机分为4组:正常组(CG组),模型组(OJ组),中药组,中药+运动组.采用改良的胆总管结扎法构建大鼠阻塞性黄疸致重症肝损伤模型,两中药组于胆总管结扎术后3天进行大柴胡汤灌胃,每天(下午6时)灌胃1次,剂量2 ml/次,共12周.运动组进行坡度5%、速度16.8 m/min、每天45 min、每周5天、共12周的中等强度跑台训练.12周后大鼠禁食过夜,随后分离出肝组织并取材,对各组大鼠肝脏组织损伤情况进行显微结构形态学观察；免疫组化法检测肝组织Toll样受体4(TLR4)及髓样分化因子88 (MyD88)蛋白表达水平；RT-PCR法检测肝组织肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、白介素10 (IL-10) mRNA表达水平；生物化学分析法检测血清谷丙转氨酶(ALT)、谷革转氨酶(AST)和总胆红素(TBIL)含量.结果:①HE染色显示:正常组肝细胞索排列整齐；模型组大鼠肝细胞大量纤维化,可见大片状肝细胞变性、坏死,乃至肝细胞索结构紊乱；中药组可见肝细胞少量点、片状坏死,肝小叶结构改变,肝索排列欠整齐；中药+运动组无明显组织坏死,肝小叶结构基本正常,肝索排列较整齐.②血清学结果:中药十运动组ALT、AST、TBIL水平较模型组显著降低(P＜0.01),中药组ALT、AST水平也显著下降(JP＜0.05).(③免疫组织化学染色结果显示:中药+运动组大鼠肝组织TLR4、MyD88表达均显著低于模型组(P＜0.01).④RT-PCR结果显示:与模型组比较,中药+运动组大鼠血清IL-6、TNF-α表达降低(P＜0.01),而IL-10表达升高(P＜0.01).结论:中等强度有氧运动联合中药大柴胡汤能调节肝脏炎症细胞因子的表达,增强阻塞性黄疸致重症肝损伤大鼠的免疫功能,从而明显减轻阻黄致大鼠肝损伤的程度.
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