利用CELⅠ酶鉴定水稻广亲和基因S5位点的籼粳属性

摘要

广亲和基因S5的克隆为水稻品种亲和特性的鉴定提供了分子学依据.利用PCR技术对水稻S5位点中籼、粳序列间存在差异的区段进行扩增,并结合芹菜核酸内切酶CEL Ⅰ对其进行酶切鉴定,从而区分S5不同的基因型.研究结果表明该方法能准确鉴定不同水稻品种中S5位点的籼粳特性,并能发现新的差异序列.%Cloning of the major locus S5. a wide-compatibility gene has laid a molecular basis for identification of the wide-compatibility varieties(WCV). According to the difference in S5 locus sequence among indica, japonica and WCV. PCR was conducted and celery endonuclease CEL I, which cleaves DNA with high specificity at sites of base-substitution mismatch, was used to digest PCR products so as to distinguish various S5 DNA fragments. The results showed that the method is accurate in indica-japonica classification. At the same time, 8 new genotype on the SS locus was found.