水稻锌指蛋白基因 OsZFP1的功能分析

摘要

生物信息学分析表明,稻瘟病菌丝氨酸蛋白酶 MoSp1在水稻中的互作蛋白 OsZfp1,为含有环指结构域的 C3 HC4型锌指蛋白.对稻瘟病菌侵染过程中 OsZFP1基因的表达动态分析表明,OsZFP1基因表达水平在稻瘟病菌 Guy11孢子悬浮液接种水稻后缓慢升高,接种后18 h 达到最高峰,约为3．8倍,这表明该基因响应稻瘟病菌的侵染.利用改进的农杆菌介导的转基因技术成功获得 OsZFP1基因的过表达植株.抗性分析表明 OsZFP1过表达植株的整体抗稻瘟病能力得到显著提高.说明 OsZFP1基因在水稻抵抗稻瘟病菌侵染过程中扮演着十分重要的角色.%The rice protein OsZfp1 which interacts with MoSp1 is a C3 HC4-type RING-finger protein predicted by bioinformatic tools.The transcriptional changes of OsZFP1 gene during Magnaporthe oryzae infection showed that the expression level of OsZFP1 peaked with 3.8 folds at 18 hours after spore-spaying inoculation,revealing that OsZFP1 might respond to M .oryzae invasion.The OsZFP1 overexpression transgenic rice plants were gained through modified Agrobacterium-mediated transformation.The statistic analysis indicated that the overexpression plants confer resistance against M .oryzae .In conclusion,the OsZFP1 gene may play an important role in rice immunity against M .oryzae .