干旱胁迫下杂草稻和栽培稻根系基因表达差异研究

摘要

The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f.spontanea ) and up-land rice IAPAR9 (Oryza sativa )were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array.The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly.For HEB07-2,among 6878 expressed genes,4693 were up-regulated and 2185 were down-regulated under drought condition.For IAPAR9,among 2923 expressed genes,983 were up-regulated and 1 940 were down-regulated.Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting (GO:000681 3 ),secondary metabolite(GO:001 9748 ),cell growth (GO:001 6049 ),glucose metabolism (GO:0006006 ),transmembrane ion transporter activity(GO:001 5075 ),ferroheme coalition(GO:0020037 ),oxidordeuctase activity(GO:001 649 1 )were significantly up-regulated in comparing with IAPAR9.These were consistent with the physiological and phenotypic data.%利用 Affymetrix 水稻表达谱芯片(GeneChip Rice Genome Array)研究抗旱杂草稻 HEB07-2与巴西陆稻(IAPAR9)在 PEG 模拟干旱胁迫下根系基因表达变化。结果表明,杂草稻 HEB07-2转录组对干旱信号的响应程度与方向均与巴西陆稻存在很大差异,HEB07-2以正向调控为主,而巴西陆稻以负调控为主。干旱胁迫下杂草稻 HEB07-2与巴西陆稻分别有6878个和2923个基因表达,其中 HEB07-2受干旱胁迫诱导上调的基因有4693个,下调的基因有2185个；而巴西陆稻则分别有983个和1940个。在差异基因表达的倍数上也是 HEB07-2高于巴西陆稻。进一步的 GO 分析表明,在干旱胁迫下, HEB07-2和 IAPAR9根系基因响应途径的差异表现为 HEB07-2在钾离子转运(GO：0006813)、次生物质代谢(GO：0019748)、细胞生长(GO：0016049)、葡萄糖代谢(GO：0006006)、跨膜离子转运器活性(GO：0015075)、亚铁血红素结合体(GO：0020037)、氧化还原酶活性(GO：0016491)等方面基因显著上调,这与生理数据和表型数据一致。