Wnt7a在红藻氨酸致痫大鼠颞叶癫痫形成过程中的变化

摘要

目的 探讨Wnt7a mRNA和蛋白在红藻氨酸(KA)致痫大鼠颞叶癫痫形成过程中的时间变化过程,明确Wnt7a是否参与颞叶癫痫海马神经发生. 方法 利用生后30天的雄性SD大鼠(哈尔滨医科大学附属第二医院动物室提供),经皮下注射KA制成颞叶癫痫动物模型后,应用Real-time PCR和Western blot技术检测KA后不同时期(急性期、自发性反复癫痫形成初期和慢性癫痫稳定形成期)实验组和生理盐水(NS)对照组(每组动物各六只)大鼠海马组织中Wnt7a mRNA和蛋白的表达. 结果 KA致痫后在颞叶癫痫形成过程中,各时期KA组动物海马组织Wnt7a mRNA的表达同相应时期NS组相比,在急性期显著下降(NS组:1.000±0.000,KA组:0.304±0.113,P＜0.001),在自发性反复癫痫形成初期增高且差异有统计学意义(NS组:1.000±0.000,KA组:3.369±1.455,P =0.010),在慢性癫痫稳定形成期增高且差异有统计学意义(NS组:1.000±0.000,KA组:5.845±3.524,P =0.020).KA组各时期的动物海马组织Wnt7a mRNA的表达相比较,癫痫形成初期及慢性期均较急性期显著增高(P＜0.05),慢性期较癫痫形成初期增高但差异无统计学意义(P＞0.05).大鼠海马组织中Wnt7a蛋白的表达无论在时间上还是在组别间差异均无统计学意义. 结论 Wnt7a可能调控KA点燃颞叶癫痫大鼠的海马神经发生,参与颞叶癫痫的形成.%Objective To explore the role of Wnt7a in the epileptogenesis by the regulation of neurogenesis in hippocampus through observation Wnt7a mRNA and protein during the process of epileptogenesis in the Kainate-induced temporal lobe epilepsy model.Methods Animal model of temporal lobe epilepsy were Male Sprague-Dawley rats (Bought for the Second Affiliated Hospital of Harbin Medical University) which induced by kainic acid (KA) at postnatal day 30,Real-time PCR method and Western blot were performed to detect Wnt7a mRNA and protein in the hippocampus of experimental and Normal Saline control groups (NS group) at different stages of epileptogensis,the acute stage,the initial stage of spontaneous recurrent seizures (SRS) and the stable stage of chronic epilepsy.Results Wnt7a mRNA decreased at the acute stage (0.304 ± 0.113,P ＜ 0.001),increased at the initial stage of SRS (3.369 ± 1.455,P =0.010)and at the stable stage of chronic epilepsy (5.845 ± 3.524,P =0.020) compared with the NS group (1.000 ± 0.000),with statistical significance.For the KA group at different stages,the expression of Wnt7a mRNA elevated significantly both at the initial stage and chronic stage (P ＜ O.05) in comparison with that at the acute stage.At the chronic stage,the expression of Wnt7a mRNA increased but the difference was not statistically significant (P ＞ 0.05) when compared with that at the initial stage.There was no significant difference of Wnt7a protein expression in the hippocampus in the time courses and the different groups.Conchsion Wnt7a may be involved in the epileptogenesis by the regulation of neurogenesis in hippocampus in kainate-induced epilepsy model.