电针对佐剂性关节炎大鼠足跖肿胀度和炎性细胞因子的影响

摘要

目的 探讨电针对佐剂性关节炎大鼠血浆内炎症相关细胞因子的作用及其与足跖肿胀度的相关性。方法 60只大鼠随机分为5组，每组12只。空白组、模型组做捆绑处理，足三里组、关元组、委中组分别于捆绑后电针单穴，隔天1次。32 d后断头取血，测量各组足跖肿胀度及血浆内白细胞介素(IL)-1、IL-2、IL-10、肿瘤坏死因子-α(TNF-α)水平。结果 造模后，模型组大鼠足跖肿胀(P＜0.05)，足三里组、关元组、委中组大鼠足跖关节肿胀度较电针治疗前改善(P＜0.05)；模型组血浆IL-1、IL-2、TNF-α含量明显高于空白组(P＜0.01)；足三里组、关元组、委中组血浆IL-1、IL-2、TNF-α含量低于模型组(P＜0.05)；委中组与足三里组、关元组有非常显著性差异(P＜0.01)；模型组血浆IL-10含量低下空白组(P＜0.05)；足三里组、关元组、委中组血浆IL-10含量高于模型组(P＜0.05)；委中组与足三里组、关元组之间有非常显著性差异(P＜0.01)；大鼠足跖肿胀度与IL-1、IL-2、TNF-α呈正相关(r=0.881～0.912，P＜0.01)；IL-1、IL-2、TNF-α之间呈正相关(P＜0.01)；IL-10与跖围、IL-1、IL-2、TNF-α呈负相关(r=-0.519～-0.293，P＜0.05)。结论 电针能够改善佐剂性关节炎大鼠足跖肿胀度，可能是通过增加血浆IL-10含量，降低IL-1、IL-2、TNF-α含量，达到改善免疫功能的作用。%Objective To explore the effect of electrical acupuncture on the cytokines in adjuvant arthritis rats. Methods 60 rats were divided into 5 groups randomly, each with 12 rats. The blank control group and the model group were only bundled; while the Zusanli group, the Guanyuan group, and the Weizhong group were electroacupunctured each single point after bundled, once per 2 d. 32 d later, the swelling of voix pedis was measured and the level of interleukin (IL)-l, IL-2, IL-10, tumor necrosis factor (TNF)-α in plasma was determined in each group. Results After being modeled, the voix pedis of rats were swelling (P<0.05), while the swelling of voix pedis in the Zusanli, Guanyuan and Weizhong groups significantly reduced (P<0.05). The plasma IL-1, IL-2 and TNF-α was significantly higher in the model group than in the blank group (P<0.01), and reduced in Zusanli, Guanyuan and Weizhong groups (P<0.05). Compared with Zusanli and Guanyuan groups, there was a significant difference in the Weizhong group (P<0.01). The plasma IL-10 was significantly lower in the model group than in the blank control group (P<0.05), and increased in the Zusanli, Guanyuan and Weizhong groups (P<0.05). Compared with Zusanli and Guanyuan groups, there was a significant difference in the Weizhong group (P<0.01). The swelling of voix pedis was positively correlated with the level of IL-1, IL-2, and TNF-a (r=0.881~0.912, P<0.01). There was positive correlation among the IL-1, IL-2, and TNF-a (P<0.01). IL-10 was negatively correlated with the swelling, IL-1, IL-2, and TNF-a (r=-0.293-0.519, P<0.05). Conclusion Elec-troacupuncture can significantly reduce the swelling of voix pedis in adjuvant arthritis rats, and it may be related with improving immune function by increasing the level of IL-10 and decreasing the level of IL-1, IL-2 and TNF-a in plasma.