大鼠颈段红核脊髓束损伤后炎症反应相关基因表达谱

摘要

目的探讨大鼠颈段红核脊髓柬(RST)损伤后急性期炎症反应的分子机制.方法 Sprague Dawley(SD)雌性大鼠18只,随机分为2组:双侧RST损伤组(n=9)和假手术对照组(n=9).制作双侧RST损伤模型,24 h后处死动物,以损伤处为中心取长约0.5 mm脊髓组织,将各组中的3只动物标本混合,提取总RNA,共得到6份RNA样品,使用含有28000个大鼠基因的Affy-metrix表达谱基因芯片检测基因变化.结果大鼠RST损伤后24 h表达发生变化的有153条基因,表达上调的有136条,表达下调的有17条.与炎症相关的基因大部分表达上调(Scn9α除外),Toll样受体信号途径中有8条基因表达上调.结论 RST损伤后多种炎症反应相关的基因表达出现变化.%Objective To investigate the characteristic changes of expression of the genes related to inflammation response after injury of rubrospinal tract(RST).Methods 18 Sprague Dawley(SD) female rats were randomly divided into 2 groups: RST injury group (n=9) and Sham group (n=9).RST injury models were established, and the rats were killed 24 hours after injury.5 mm length spinal cord was harvested from the epicenter and total RNA was extracted.Affymetrics Gene Chips for rats, representing 28000 genes, were used for mRNA expression profiling.Results 153 transcripts were observed to differ (2.0 fold; 136 up-regulated and 17 down-regulated) after injury of RST, compared with sham group.Most of genes related to inflammation response were upregulated (except Scn9α).8 genes related to Toil-like receptor signaling pathway were also up-regulated.Conclusion Significant changes related to inflammation response occur in acute phase after injury of RST.