鸡IL-1β、IL-16基因真核表达质粒对IBV DNA疫苗的免疫增强效应研究

摘要

To enhance the immune efficacy of infectious bronchitis virus (IBV) DNA vaccine, eukaryotic recombinant plasmids expressing IL-1β and IL-16 of Sichuan Mountainous Black-bone chicken, pDNAIL-1β and pDNAIL-16, were constructed and utilized to co-inoculate 7-day-old SPF chicken with IBV DNA vaccine as molecular adjuvants. Five weeks post inoculation, chickens were challenged by virulent IBV strain. The co-inoculation of either molecular adjuvants induced a higher level of specific antibody and the ratio of CD3+, CD4+ and CD8+ T lymphocytes in perpheral blood, and provided an higher protection rate (25％ or 15％) against the virulent IBV strain than the group inoculated only with DNA vaccine. Moreover, the enhancing effect of pDNAIL-1β was found to last at least 7 day longer than that of pDNAIL-16. These results indicated that pDNAIL1β and pDNAIL16 could be used to improve the efficacy of IBV DNA vaccine through co-administration with the vaccine.%为研究鸡IL-1β、IL-16基因真核表达质粒对鸡传染性支气管炎病毒(my)DNA疫苗免疫效果的影响,根据GenBank登录的鸡IL-1β和IL-16序列设计两对引物,采用RT-PCR方法从四川山地乌骨鸡外周血淋巴细胞RNA中克隆IL-1β和IL-16基因,与真核表达质粒pcDNA3.1(+)连接构建了pDNAIL-1β和pDNAIL-16.分别将两种重组质粒与IBV DNA联合免疫SPF雏鸡并进行攻毒试验.结果表明,pDNAIL-1β和pDNAIL-16均能促进血清中特异性抗体水平和外周血T淋巴细胞亚群数量的增加(p<0.05),增强IBV DNA疫苗对同型强毒的保护率15%～25%,pDNAIL-1β提升DNA疫苗的抗体水平的时间比pDNAIL-16多7 d.试验表明,鸡IL-1β、IL-16基因真核表达质粒对IBV DNA疫苗的免疫具有增强作用.