非小细胞肺癌组织中Survivin与Fas/FasL的表达及其临床意义

摘要

目的 检测非小细胞肺癌(NSCLC)组织中Survivin、Fas/FasL的表达.方法 采用免疫组织化学法检测16例正常肺组织、48例肺鳞癌组织、38例肺腺癌组织中Survivin、Fas/FasL的表达.结果 正常肺组织中Survivin的阳性表达率为0,而在肺鳞癌和肺腺癌组织中Survivin的阳性表达率分别为79.17%和70.05%,与正常肺组织相比二者差异均有统计学意义(P<0.01);正常肺组织中Fas的阳性表达率为81.25%,与肺鳞癌(41.67%)和肺腺癌(43.37%)相比,差异均有统计学意义(P<0.05);Fas的表达与性别、组织学类型、TNM分期及有无淋巴结转移均无相关性.正常肺组织中FasL的阳性表达率为18.75%,低于肺鳞癌(72.92%)和肺腺癌(68.42%),P<0.05.NSCLC组织中FasL的表达与性别及组织学类型无关,与TNM分期和淋巴结转移有关.结论 Survivin基因异常表达而引起的细胞凋亡抑制在NSCLC的发生中起一定的作用,其过度表达提示NSCLC预后不良;NSCLC Fas低表达在其发病中可能有一定的作用;Fas/FasL可能参与了NSCLC凋亡的调节,在NSCLC逃逸机体免疫监控方面起重要作用,因此检测FasL的表达可能为临床判断肺癌淋巴结转移提供依据.%Objective To detect the expression of Survivin, Fas/FasL in non-small cell lung cancer(NSCLC) tissue. Methods We used immunohistochemistry to detect the expression of Survivin, Fas/FasL in 16 cases of normal lung, 48 cases of squamous carcinoma of lung tissue, 38 cases of adenocarcinoma of lung tissue. Results The positive rate of Survivin in normal lung tissue, squamous carcinoma and adenocarcinoma of lung were 0, 79.17% and 70.05% respectively, and there was significant difference between the normal and the two NSCLC groups(P<0.01). The positive rate of Fas in normal lung tissue(81.25%) was significantly higher than that in squamous carcinoma of lung(41.76%) and adenocarcinoma of lung(43.37%),P<0.05. The positive of FasL in normal lung tissue(18.75%) was significantly lower than that in SqCa(72.92%) and adenocarcinoma of lung(68.42%)(P<0.05). No relation was found between the expression of FasL and clinicopathologic factors including sex, histoclassification. And there was a relation between lymphnode metastasis and TNM stage of NSCLC. Conclusions Apoptosis inhibitition caused by abnormal survivin expression may participate in the onset and progression of NSCLC. The over-expression survivin suggests the bad prognosis in NSCLC. The expression of Fas protein in NSCLC was low, and might play a role in carcinogenesis of lung carcinoma. The expression of Fas/FasL in NSCLC may play an important role in the escape of tumor cells from immune function. The expression of FasL might be used as an objective indicator of metastasis potency of local lymph node.