卵巢癌中CD44 nm23基因及细胞因子rn TGFα TNFα表达的研究

摘要

Objective To explore the relation of the expression of intron 9 in CD44 gene,nm23 gene and the serum levels of TGFα and TNFα to the development, metastasis and prognosis of epithelial ovarian cancer. Methods The expressions of intron 9 in CD44 gene, nm23 gene, TGFα and TNFα in epithelial ovarian cancer were measured by semi -quantitative RT - PCR, immunohistochemical technique and radioimmunoassay ( RIA ). Results There was positive expression of intron 9 in CD44 gene,nm23 gene,TGFα and TNFα in 36 cases of epithelial ovarian cancer. The higher levels of nm23 gene and TGFa were obvious in early stage of ovarian cancer. Conclusion To study the ovarian cancers biologic behavior with these multipe factors can provide theoretical basis for early diagnosis, gene therapy and predicting prognosis.%目的探讨细胞表面粘附分子CD44基因、肿瘤转移抑制基因nm23、转化生长因子α(TGFα)、肿瘤坏死因子α(TNFo)与卵巢癌发生、转移和预后的关系。方法利用PCR半定量、免疫组化及放免法分别测定36例卵巢癌及32例卵巢良性肿瘤的CD44基因内含子9、肿瘤转移抑制基因nm23、转化生长因子α和肿瘤坏死因子α的表达情况。结果 CD44基因内含子9、nm23、TGFα、TNFα在36例卵巢癌中均出现高表达，nm23和TGFα在癌症早期阶段即出现高表达。结论多相关因素分析卵巢癌的生物学行为，可为卵巢早期诊断、基因治疗及预后判断提供客观理论依据。