人乳头瘤病毒亚型与宫颈上皮内瘤变的相关性

摘要

Objective To investigate the relationship of the infection of subtype or co-infection of multi-subtype of human papillomavirus (HPV) with cervical intraepithelial neoplasia( CIN). Metheds From november,2007 to april,2008 in the general hospital of Tianjin medical university, the detection of HPV subtype were tested by polymerase chain reaction ( PCR),DNA gene chip probe hybridization. All the cases was divided into two groups accroding to their age, lower or e-qual to 35 years is divided into group A,older than 35 years is divided into group B. Results The incidence of HPV infection in total 98 cases is 72.41% (21/29) of CIN I ,75% (27/36) of CIN II ,96. 97% (32/33) of CINI. Sigle HPV subtype infection in CIN I ,CIN II and CIN III is 41. 38% ( 12/29) ,44. 44% (16/36) and 57. 58% ( 19/33) respectively ; double HPV subtype infection in CIN I , CIN II and CIN HI is 27. 59% (8/29 ) , 19. 44% ( 7/36) and 27. 27% (9/33 ) respectively; multiple HPV subtype infection in CIN I , CIN II and CIN I is 3.45% (1/29), 11. 11% (4/36) and 12.12% (4/33) respectively. The main subtype infected in CIN I was lower risk HPV( LR-HPV), while in CIN I , HPV16 being the dominant. The incidence of HPV16 infection in group A and B is 20.93% ( 9/43), 40% (22/55) respectively ; and the difference is significant (P < 0. 05). Conclusion The incidence of HPV infection rise with the progress of the grade of CIN.companied by rising trence of double or multiple HPV infection. The subtype of HPV infected in CIN DI is different with the progress of the disease. The main subtypes of HPV infected in CIN II is HPV 16 and HPV58. The double or multiple HPV infection may promote the progress of CIN. It will greatly reduce the incidence of CIN HI or inversive cervical cancer to prevent or curb the infection of HPV16 and HPV58.%目的 探讨人乳头瘤病毒( HPV)各亚型感染或多亚型感染与宫颈上皮内瘤变(CIN)的相关性.方法 2007年11月至2008年4月在天津医科大学总医院采用PCR扩增、基因芯片探针杂交分型检测法检测98例CIN患者的HPV亚型.以35岁为界将98例CIN患者分为两组,年龄≤35岁者为A组（43例）；年龄＞35岁者为B组（55例）.结果 98例CIN中HPV阳性率,CIN Ⅰ为72.41％ (21/29),CINⅡ为75％ (27/36),CINⅢ为96.97％ (32/33).HPV单重感染率在CIN Ⅰ、CINⅡ和CINⅢ中分别为41.38％ (12/29),44.44％( 16/36)和57.58％ (19/33)；HPV双重感染率在CIN Ⅰ、CINⅡ和CINⅢ中分别为27.59％ (8/29),19.44％ (7/36)和27.27％(9/33)；HPV多重感染率在CIN Ⅰ、CINⅡ和CINⅢ中分别为3.45％( 1/29),11.11％ (4/36)和12.12％ (4/33).CIN Ⅰ中主要为低危型HPV感染,CINⅢ中以HPV16为主导感染亚型.AB两组HPV16感染率分别为20.93％(9/43),40％ (22/55),差异具有统计学意义(P＜0.05).结论 随CIN病情发展和病变严重,HPV感染率逐渐升高,且双重感染、多重感染均呈升高趋势；HPV感染的亚型不同.CINⅢ中的主要HPV亚型为HPV16、58；HPV的双重或多重感染可能促进了CIN的进程.预防或控制HPV16、58感染,将大大减少CINⅢ及宫颈浸润癌的发生.