Lx2-32c对宫颈癌细胞Hela影响的体外研究

摘要

Objective To study the effects of Lx2-32c on the proliferation and apoptosis of cervical cell Hela.Methods After treated with different concentration of Lx2-32c for 24,48,72 h,the proliferation of cervical cell Hela was determined by MTT.Simultaneously,the apoptosis ratio of Hela by Annexin V-FITC/PI and the expression of Bcl-2 and cleaved-caspase-3 by western blotting were detected.Results Lx2-32c could effectively inhibit the proliferation of Hela in a dose-dependent and time-dependent manner.At the same time,Lx2-32c induced apoptosis and down-regulated the expression of Bcl-2 and up-regulated the expression of cleaved-caspase-3 in Hela.Conclusion Lx2-32c maybe induced apoptosis and inhibited the proliferation of Hela involving in mitochondrial pathway.%目的 观察Lx2-32c对宫颈癌细胞Hela生长增殖、凋亡的影响.方法 以不同浓度的Lx2-32c处理宫颈癌细胞Hela 24、48和72 h后,采用MTT法检测各浓度对细胞生存率的影响,计算细胞增殖活力,同时以Annexin V-FITC/PI双染法测定不同浓度的Lx2-32c诱导Hela细胞发生凋亡的情况,并检测Bcl-2与cleaved-caspase-3的表达.结果 不同浓度的Lx2-32c能够有效的抑制Hela的生长增殖,随着时间延长,其抑制作用更加明显,呈时间依赖性与浓度依赖性,并能够有效的诱导Hela细胞发生凋亡.Western blot结果显示,Lx2-32c能够有效降低Hela细胞中Bcl-2表达,同时上调cleaved-caspase-3的表达.结论 Lx2-32c可能通过线粒体通路诱导宫颈癌细胞Hela发生凋亡和抑制生长增殖.