直肠粘膜吸引活检免疫组织化学法诊断先天性巨结肠症

摘要

本文采用神经元特异性稀醇化酶(N S E)免疫组织化学方法,对32例怀疑为先天性巨结肠症患儿的直肠粘膜吸引活检标本进行检查,无一例误诊.我们认为常规组织学方法或乙酰胆碱酯酶组织化学方法不能确定诊断时,采用NSE免疫组织化学方法是较好的选择.%Thirty two children SUSpected Hirschs prung's disease(HD)were examined by neuronsoecific-enolase(NSE)immunohistochemical ABC method.The specimens were got by rectal suction biopsy.The diagnosis of HD was established in 27 patients and excluded in 5.In all patients,the diagnosis is confirmed later either by other co-examinations or by operations.There was no misdiagnosis in our series.The ganglion ceils and neural fibers in submucosa are readily stained with NSE metbod and can easily be identified,especially in the neonates whose submucosal ganglion cells are immnture.NSE immunohistochemical method is a good choice when the diagnosis can't be concluded by HE stain or AChE stain.