人胚胎成纤维细胞对人胚胎干细胞生长的作用

摘要

AIM: In order to replace mouse embryonic fibroblast cells byhuman embryonic fibroblast cells to support the growth of human embryonic stem cells, the effects of human/mouse embryonic fibroblast cells on the growth of human embryonic stem cells were compared. METHODS: Both mouse and human embryonic fibroblast cells were used as feeder layer to support human embryonic stem cells. The proliferation and differentiation of human embryonic cells were observed. RESULTS: Combined use of human leukemia inhibitory factor with human/mouse feeder layer cells would support growth and proliferation of human embryonic stem cells and kept in undifferentiated condition. There was no difference between human/mouse cell. CONCLUSION: Human embryonic fibroblast cells can be used to support proliferation of human embryonic stem cells, eliminating the influence of foreign protein.%目的：比较人和小鼠胚胎成纤维细胞对人胚胎干细胞生长的作用，为胚胎干细胞定向诱导各系统细胞应用于临床，消除异种蛋白污染打下基础。方法：分别采用人胚胎成纤维细胞和小鼠胚胎成纤维细胞为饲养层细胞,支持人受精卵的培养，观察其增殖和分化情况。结果：人和小鼠胚胎成纤维细胞分别加入白血病抑制因子（hLIF）均能很好支持人胚胎干细胞生长增殖，并保持未分化状态。结论：完全可以使用人胚胎成纤维细胞支持人胚胎干细胞增殖，消除异种蛋白污染的可能性，为胚胎干细胞定向诱导分化发育应用于临床打下坚实基础