Nodosin对HepG2细胞株的增殖抑制作用及对Bcl-2和Bax表达的影响

摘要

目的：研究传统中药提取物nodosin对体外培养的人肝癌细胞株HepG2的增殖抑制作用，并探讨其对Bcl-2和Bax表达的影响。方法：将不同浓度组（1．25、2．5、5、10和20μmol/L） nodosin预作用于HepG2细胞24 h，用倒置显微镜观察nodosin对细胞形态学的影响，采用MTT法检测细胞生长抑制率，用流式细胞术检测细胞凋亡率和Bcl-2、Bax的表达。结果：形态学结果显示，随着nodosin剂量的增加，细胞皱缩和漂浮细胞数量增加，流式细胞术检测结果显示随着nodosin药物浓度的增加，HepG2细胞凋亡率增加。 Bax的表达随着nodosin剂量的增加逐渐增加， Bcl-2的表达逐渐减少。结论：Nodosin能抑制HepG2细胞株的增殖，呈明显的剂量依赖性，对HepG2细胞的抑制作用是通过增加Bax的表达以及降低Bcl-2的表达诱导细胞凋亡实现的。%AIM:To investigate the effects of nodosin extracted from Chinese traditional medicine on the pro-liferation of HepG2 cells cultured in vitro and to detect the protein expression of Bcl-2 and Bax in HepG2 cells.METH-ODS:HepG2 cells were treated with different concentrations (1.25, 2.5, 5, 10 and 20 μmol/L) of nodosin for 24 h. The morphological changes of HepG2 cells were observed under inverted microscope.The inhibitory rates of HepG2 cell growth were detected by MTT assay.The apoptotic rates and the protein expression of Bcl-2 and Bax were analyzed by flow cytometry.RESULTS:Shrunken and suspended HepG2 cells increased with the increases in the concentrations of nodo-sin.The apoptotic rates and the expression of Bax increased with the increases in the doses of nodosin, while the expression of Bcl-2 decreased.CONCLUSION:Nodosin inhibits the growth of HepG2 cells in a dose-dependent manner.The inhibi-tion of HepG2 cell growth is induced by decreasing Bcl-2 and increasing Bax, thus promoting cell apoptosis.