AIM:To investigate the effects of neuregulin-1 (NRG-1) on the expression of angiogenic factors in human coronary artery smooth muscle cells ( HCASMCs) . METHODS:HCASMCs were cultured in vitro, and the cells at the 3rd passage were collected to assess the expression and phosphorylation of ErbB by Western blot. After HCASMCs were cultured under normal condition, with hypoxia and serum deprivation, or with NRG-1 treatment, the expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) was determined by Western blot. RESULTS:The expression of ErbB2, ErbB3 and ErbB4 was observed in the HCASMCs, and the phosphorylation of these receptors was increased by NRG-1 treatment. Compared with control group, the expression of VEGF and Ang-1 in the HCASMCs was significantly increased in hypoxia and serum deprivation group (P<0. 05), while no difference in the ex-pression of Ang-2 between the 2 groups was found. Compared with hypoxia and serum deprivation group, the expression of VEGF and Ang-1 in the HCASMCs treated with NRG-1 was further increased (P<0. 05), and no difference in the expres-sion of Ang-2 between the 2 groups was observed. CONCLUSION: HCASMCs express ErbB2, ErbB3 and ErbB4, and the phosphorylation of the receptors is increased by NRG-1. Hypoxia, serum deprivation and NRG-1 treatment induce the increased expression of VEGF and Ang-1 significantly.%目的：：探讨神经调节蛋白1(neuregulin-1,NRG-1)对人冠状动脉平滑肌细胞(HCASMCs)表达血管生成因子的影响。方法：培养HCASMCs,实验使用第3代细胞。用Western blot法检测细胞ErbB的表达和磷酸化。在正常、缺氧缺血清或NRG-1(100μg/L)处理条件下,用Western blot法检测血管内皮生长因子(VEGF)、血管生成素1(Ang-1)和血管生成素2(Ang-2)表达的改变。结果： ErbB2、ErbB3和ErbB4均能在HCASMCs中表达,加入NRG-1后,这3种ErbB的磷酸化水平均增加。与对照组比较,在缺氧缺血清组HCASMCs中VEGF和Ang-1的表达明显增加(P <0.05),而 Ang-2的表达差异无统计学显著性。与缺氧缺血清组比较,NRG-1处理组的HCASMCs表达VEGF和 Ang-1进一步显著增加(P <0.05),而 Ang-2的表达差异无统计学显著性。结论：HCASMCs能表达ErbB2、ErbB3和ErbB4,加入NRG-1增强ErbB2、ErbB3和ErbB4的磷酸化。缺氧缺血清和NRG-1处理均能增加VEGF和Ang-1在HCASMCs中的表达。
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