miR-153靶向PRDM2基因并通过JAK/STAT信号通路影响膀胱癌的侵袭和迁移

摘要

目的:研究微小RNA(miR)-153是否靶向正性调控域锌指蛋白2(PRDM2)基因并通过调控JAK/STAT信号通路影响膀胱癌细胞的侵袭和迁移.方法:运用qPCR检测miR-153在膀胱癌组织中的表达;运用免疫组化检测PRDM2在正常组织和膀胱癌组织中的表达;Western blot检测不同膀胱癌细胞株中PRDM2的表达情况;双萤光素酶报告基因系统检测miR-153对PRDM2转录活性的影响;Transwell侵袭实验检测miR-153过表达对膀胱癌细胞RT4侵袭能力的影响;划痕实验检测miR-153过表达对膀胱癌细胞RT4迁移能力的影响;Western blot实验检测过表达miR-153后JAK/STAT信号通路蛋白的水平.结果:和正常组织比较,PRDM2蛋白在膀胱癌中表达较高(P<0.05);miR-153的表达水平在膀胱癌组织中较低(P<0.05);双荧光素酶报告基因系统检测结果显示,miR-153可以调控PRDM2的表达水平;过表达miR-153后,膀胱癌细胞株RT4的侵袭和迁移能力明显降低,p-JAK2和p-STAT3的蛋白水平下调.结论:miR-153靶向PRDM2,并通过JAK/STAT信号通路调控膀胱癌细胞的侵袭和迁移能力.%AIM:To investigate the effect of microRNA(miR)-153-targeted positive regulatory domain zinc finger protein 2(PRDM2 )on invasion and migration abilities of bladder cancer cells through JAK /STAT signaling path-way.METHODS:The expression levels of miR-153 in bladder cancer tissues were detected by qPCR.The expression of PRDM2 in normal tissues and bladder cancer tissues was detected by immunohistochemistry.The effect of miR-153 on the transcriptional activity of PRDM2 was examined by dual-luciferase reporter assay system.The effect of miR-153 over-ex-pression on the invasion ability of bladder cancer RT 4 cells was detected by Transwell assay ,and the cell invasion ability was analyzed by scratch test.The protein levels of PRDM2,p-JAK2 and p-STAT3 were determined by Western blot.RE-SULTS:The expression of miR-153 was significantly lower in the bladder cancer tissues than that in normal tissues(P<0.05).PRDM2 was highly expressed in the bladder cancer tissue(P<0.05).The results of dual-luciferase reporter assay system showed that miR-153 regulated the expression of PRDM2.miR-153 over-expression significantly decreased the invasion and migration abilities of bladder cancer RT 4 cells.miR-153 over-expression also down-regulated the protein levels of p-JAK2 and p-STAT3(P<0.05).CONCLUSION:miR-153 targets PRDM2,and regulates the invasion and migra-tion abilities of bladder cancer cells by JAK/STAT signaling pathway.