目的 探讨白细胞介素10(IL-10)启动子区-1082/-819/-592位点的多态性及其在血浆中的浓度与声带白斑发生的关系.方法 选择2012年10月至2013年2月61例声带白斑患者和119名正常人作对照研究,采用核苷酸直接测序法检测样本的IL-10基因位点(-1082G/A,-819C/T和-592C/A)的基因分型,用酶联免疫吸附法(ELISA)检测IL-10的血浆浓度.结果 吸烟和饮酒增加了罹患声带白斑的风险(OR为4.73和5.70,95％ CI为2.4 ～9.1;2.9～11.2,P＜0.01).声带白斑患者在IL-10的-592和-819位点比对照组有更高的AC突变频率(OR=1.93,95％ CI为0.97～ 3.81,P=0.05),在-1082位点有更高的AG突变频率(OR =2.14,95％ CI为0.87 ～5.27,P=0.092);声带白斑患者的血浆IL-10质量浓度为(21.6 ±0.5)pg/ml((x)±s),对照组为(19.0±1.1) pg/ml,两者比较差异有统计学意义(t=2.08,P=0.043).基因型中含有G基因的单倍体患者的血浆IL-10质量浓度(24.3±5.7)pg/ml,比正常基因型单倍体患者IL-10的浓度高(19.9 ±4.7) pg/ml,两者比较差异有统计学意义(t=-2.64,P=0.008).结论 IL-10启动子区-1082/-819/-592多态性及血浆中高浓度的IL-10与声带白斑的发生有关,同时,突变基因型与IL-10血浆浓度有关.%Objective To investigated the association between interleukin-10(IL-10)-1082/-819/-592 promoter polymorphism,plasma IL-10 levels and risk of vocal leukoplakia.Methods A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed.Genotypes for the IL-10 gene (IL-10-1082 G/A,-819 C/T and-592 C/A) were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA.Results Smoking and alcohol consumption increased the risk of vocal leukoplakia (OR =4.73,95％ CI:2.4-9.1 ; OR =5.70,95％ CI:2.9-11.2,P ＜ 0.01).Patients with vocal cord leukoplakia had significantly higher frequencies of AC at-592 and-819 (OR =1.93,95％ CI:0.97-3.81,P=0.05) and AG at-1082(OR =2.14,95％ CI:0.87-5.27,P =0.092) than control.Vocal leukoplakiapatients had higher concentration of plasma IL-10 (21.6 ± 0.5)pg/ml ((x) ± s) than controls (19.0 ± 1.1) pg/ml(t =2.08,P =0.043) and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations (24.3 ± 5.7) pg/ml compared with the ATA haplotype (19.9 ± 4.7)pg/ml (t =-2.64,P =0.008).Conclusions IL-10-1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia,and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism.
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