γδ T细胞在慢性鼻-鼻窦炎鼻黏膜组织中的表达及意义

摘要

Objective To explore the expression of γδ T cells in chronic rhinosinusitis (CRS) and its potential significance in pathogenesis.Methods γδ T cell expression was detected by immunohistochemistry (Envision method).From polyps (25 CRS patients with nasal polyps,CRSwNP),inferior turbinate mucosa (13 CRS patients without nasal polyps,CRSsNP),and 16 inferior turbinate mucosa from patients with deviation of nasal septum served as control.The infiltration of eosinophils in eosinophilic CRSwNP was observed by HE staining.The differences of expression of γδ T cells between each groups were compared,meanwhile the relationship between γδ T cells and eosinophils were analyzed.SPSS 16.0 software was used to analyze the data.Results The positive range of γδ T cells in CRSwNP group and CRSsNP group was 88.0％ and 84.6％,respectively,both higher than 37.5％ in control group (x2 =13.413,P ＜ 0.01,x2 =6.564,P ＜ 0.05,respectively),CRSwNP group had no statistical significance compared with CRSsNP group(x2=0.086,P ＞0.05).The expression of γδ T cells in CRSwNP group was stronger than CRSsNP group and control group (U =596,P ＜ 0.01,U =296,P ＜ 0.01,respectively) ; CRSsNP group was stronger than control group(U =216,P ＜ 0.05).There was a positive correlation between γδ T cells and eosinophils (r =0.579,P ＜ 0.05).Conclusions The expression of γδ T cells was increased in nasal mucosa of CRS.γδ T cells may be involved in the pathogenesis of CRS.%目的 观察γδ T细胞在慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)患者鼻黏膜组织中的表达,探讨其在CRS发病机制中的意义.方法 免疫组化(Envision二步法)分别检测CRS伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者息肉组织(25例)、CRS不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者下鼻甲黏膜(13例)及对照组鼻中隔偏曲患者下鼻甲黏膜(16例)中γδ T细胞的表达情况；HE染色观察嗜酸粒细胞浸润型CRSwNP中嗜酸粒细胞浸润程度.比较各组间γδ T细胞表达差异,分析嗜酸粒细胞浸润型CRSwNP中γδ T细胞表达与嗜酸粒细胞浸润程度的相关性.采用SPSS 16.0软件对数据进行分析.结果 CRSwNP组和CRSsNP组中yδT细胞阳性率分别为88.0％、84.6％,均高于对照组的37.5％,差异有统计学意义(x2=13.413,P＜0.01；x2=6.564,P＜0.05).CRSwNP组与CRSsNP组相比,γδ T细胞阳性率差异无统计学意义(x2=0.086,P＞0.05).CRSwNP组中γδ T细胞表达程度高于CRSsNP组和对照组(U值分别为596、296,P值均＜0.01),CRSsNP组γδ T细胞表达程度高于对照组(U=216,P＜0.05).CRSwNP组中γδT细胞表达与嗜酸粒细胞浸润程度呈正相关趋势(r=0.579,P＜0.05).结论 γδ T细胞在CRS中表达增高,并可能促进鼻黏膜组织中嗜酸粒细胞浸润,可能在CRS发病机制中发挥作用.