白藜芦醇灌胃给药对大鼠脊髓损伤炎症反应中 MPO和 CCO 的作用

摘要

Objective To investigate the effect of intragastric resveratrol on the activity of the myeloperoxidase ( MPO ) and cytochrome C oxidase ( CCO) in inflammatory reaction after the spinal cord injury in rats.Methods A total of 96 adult male Sprague-Dawley rats, which were selected from February 2008 to February 2012 in the Animal Department of People’ s Hospital of Wuhan University, were randomly divided into 4 groups.The model of moderate spinal cord injury was established according to Allen’s technique.Rats were fed with resveratrol (100 mg/kg) or methylprednisolone sodium (MPSS, 100 mg/kg) through the tube.At the 8th h, the 1st d, the 3rd d, and the 7th d after the spinal cord injury, the changes of MPO and CCO activity were observed using colorimetry and compared between the two groups.Results The difference of the activity of MPO and CCO between the two groups was significant at the 8th h, the 1st d, the 3rd d, and the 7th d after the spinal cord injury (P<0.01), indicating an protective effect of resveratrol on nerve cells after the spinal cord injury.Moreover, the difference of the effect between the resveratrol group and the MPSS group was observed at the 1st d after the spinal cord injury ( P <0.01 ).Conclusion Resveratrol can protect the nerval cells after the spinal cord injury by inhibiting the activity of MPO and improving the activity of CCO .%目的：探讨白藜芦醇灌胃（管饲）后对脊髓损伤后炎症反应中的髓过氧化物酶和细胞色素C氧化酶活性的影响。方法2008年2月至2012年2月在武汉大学人民医院动物实验中心将96只Sprague-Dawley健康成年雄性大鼠随机分成4组，根据Allen’ s法制成中度脊髓损伤模型，术后按照100 mg/kg立即管饲白藜芦醇或甲基强的松龙；通过比色法观察脊髓损伤8 h，1 d，3 d及7 d后白藜芦醇组脊髓髓过氧化物酶和细胞色素C氧化酶活性的变化，并与甲基强的松龙组进行疗效对比。结果在脊髓损伤管饲后8 h，1 d，3 d及7 d白藜芦醇组髓过氧化物酶和细胞色素C氧化酶活性与损伤组统计学上具有显著性差异（ P＜0.01），显示白藜芦醇管饲后对脊髓损伤具有明显神经细胞保护作用，而且损伤后1d白藜芦醇组与甲基强的松龙组间统计学上具有显著性差异（ P＜0.01）；结论在脊髓损伤后管饲白藜芦醇，能够有效降低髓过氧化物酶活性的升高幅度，并提高细胞色素C氧化酶活性，对损伤后脊髓起保护作用。