碱性成纤维细胞生长因子对人晶状体上皮细胞增殖及移行作用的影响

摘要

目的 探讨碱性成纤维细胞生长因子(bFGF)对体外培养的人晶状体上皮细胞(HLEC)增殖及移行作用的影响.方法 在无血清培养液培养的HLEC中分别加入不同终浓度的bFCF(0.01、0.1、1、10及100 μg/L),MTT法测定其促细胞增殖的情况;流式细胞仪分析细胞周期;HLEC损伤愈合模型,观察不同终浓度bFGF处理24 h后HLEC的移行情况.结果 bFGF浓度为0.1、1、10、100 μg/L时,其对HLEC细胞有明显的促增殖作用,与阴性对照组比较差异具有统计学意义(P<0.01),100 μg/L作用24 h增殖率最大,达112.78%,作用强度呈浓度时间依赖性.bFGF通过促进细胞周期变化,与阴性对照组比较差异具有统计学意义(P<0.01),G0/G1期细胞减少,S期和G2/M期细胞增多,通过促进HLEC由G0向G1的转化来促进细胞的增殖;bFGF浓度1、10、100 μg/L作用24 h.可明显促进HLEC的移行,其移行能力分别为27.21%、154.42%、和238.77%,与阴性对照组相比,差异有显著统计学意义(P<0.01).结论 bFGF可促进HLEC的增殖和移行,是HLEC强有力的有丝分裂原和促移行因子.%Objective To investigate the effect of basic fibroblast growth factor (bFGF) on proliferation and migration in cultured human lens epithelial cell (HLEC). Methods HLEC cultured in a serum-free medium were treated with bFGF (0.01, 0.1, 1, 10 and 100 μg/L). MTT assay was used to detect the proliferation effect and a FACS machine was used to detect the cell cycle. An in vitro wound healing model was used to detect the migration of HLEC treated with bFGF (0.01, 0.1, 1, 10 and 100 μg/L) after 24 hours. Results bFGF (0.1, 1, 10, 100 μg/L) increased proliferation rates of cultured HLEC with density/time dependence. Compared to the control group, there was a significant difference (P<0.01). The addition of bFGF at a concentration of 100 μg/L for 24 hours was the maximal increase in the proliferation rate (112.78%). bFGF also induced proliferation by stimulating the G0/G1 phase cell decrease and increasing the S phase and G2/M phase. bFGF showed effects on migration of 27.21%, 154.42%, and 238.77% at concentrations of 1 μg/L, 10 μg/L. 100 μg/L, respectively. Compared to the control group, there was a significant difference (P <0.01). Conclusion bFGF can induce the proliferation and obvious migration of HLEC; consequently, bFGF is a mitogen and potent migratory factor for HLEC.