目的 探讨pEGFP-ING4对裸鼠体内人U87细胞移植瘤生长及对肿瘤血管生成的影响.方法 将成功构建的18只人U87细胞裸鼠皮下移植瘤模型,随机分为pEGFP-ING4和pEGFP-C2转染组及PBS空白对照组3组,测量各组肿瘤的体积变化,荧光显微镜观察转染瘤体内绿色荧光蛋白(green fluorescent protein,GFP)的表达情况,免疫组织化学SABC法检测微血管密度(microvessel density,MVD).结果 荧光显微镜下pEGFP-ING4 组和pEGFP-C2组均观察到GFP表达,接种后第21d、28d、35d各组皮下肿瘤体积(mm3)为:PBS 组(201.8±19.3,418.9±26.4,622.1±51.3),pEGFP-C2 组(197.6±18.9,398.4±20.4,593.7±48.7),pEGFP-ING4 组(138.9±8.4,198.7±21.5,293.2±31.6),肿瘤接种后在同一时间点内,pEGFP-ING4 组肿瘤体积明显小于另外两组(P<0.05);MVD检测(个/mm2):PBS 组(15.83±0.98),pEGFP-C2 组(15.83±1.62),pEGFP-ING4 组(4.17±1.17),与另外两组比较,pEGFP-ING4 组MVD明显降低(P<0.01).结论 ING4基因能够明显抑制人U87细胞裸鼠皮下移植瘤的生长,抑制胶质瘤血管的生成可能是其抗肿瘤的重要机理之一.%Objective To study the effects of pEGFP - ING4 on the growth and angiogenesis of human U87 cells xenografts in nude mice. Methods The xenografts derived from U87 cells were established in 18 BALB/C nude mice. Inoculated mice were randomly divided into 3 groups: pEGFP - ING4, pEGFP - C2 and PBS groups. Tumor volume was measured and microvessel density ( MVD) were detected using SABC immunohistochemistry. The expression of green fluorescent protein ( GFP) was detected using a fluorescent microscope. Results GFP could be detected in pEGFP - ING4and pEGFP - C2 groups but not in PBS group undermicroscope. After tumor inoculation, the 2lth .28 th .35 th day the volume ( mm3 ) of each group is : PBS group ( 201. 8 ± 19. 3 .418. 9 ± 26. 4 , 622. I ±51. 3) , pEGFP - C2 group ( 197. 6 ±18. 9 .398. 4 ± 20. 4 ,593. 7 ±48. 7) ,pEGFP - ING4 group( 138. 9 ± 8. 4 , 198. 7 ± 21. 5 , 293. 2 ± 31. 6) . Compared with the other two groups at the same time , pEGFP - ING4 group was suppressed significantly(ρ＜0. 05) . The MVD was : PBS group( 15. 83 ±0. 98) , pEGFP - C2 group ( 15. 83 ± 1. 62 ) , pEGFP - ING4 group ( 4. 17 ± 1. 17 ), the MVD in pEGFP ING4 group was significant reduction(ρ＜0. 01) compared with the other two groups. Conclusions ING4 gene can inhibit the growth of U87 cell xenografts in nude mouse. The inhihition of angiogenesis might play a key role in the antitumor effect of INGA.
展开▼
