IMP3、IGF2在人脑胶质瘤中的表达及与预后的关系

摘要

目的 探讨胰岛素样生长因子IImRNA结合蛋白3(insulin-like growth factor-Ⅱ mRNA-binding pro?tein 3,IMP3)及胰岛素样生长因子-2(Insulin-like growth factor-2,IGF2)在胶质瘤中的表达及对临床预后的影响.方法 采用免疫组化S-P法检测171例脑胶质瘤组织及20例非瘤脑组织中IMP3、IGF2及Ki-67的表达情况;分析IMP3、IGF2及Ki-67表达强度间的相关性;Kaplan-Meier生存分析及多变量Cox比例风险回归模型分析胶质瘤患者生存状况的影响因素.结果 非瘤脑组织、胶质瘤组织中IMP3、IGF2和Ki-67的表达水平差异均有统计学意义( P均<0.05).且胶质瘤组织中IMP3、IGF2蛋白及Ki-67表达均随肿瘤病理级别升高而增高(P均<0.05).Spearman相关分析显示,IMP3、IGF2与Ki-67表达水平皆呈正相关,且IMP3蛋白的表达与IGF2蛋白的表达水平呈正相关(P均<0.05).Kaplan-Meier分析结果显示IMP3、IGF2的表达与临床预后密切相关(P<0.05).Cox比例风险回归模型分析示,肿瘤病理分级、IMP3、IGF2及Ki-67均为胶质瘤患者预后的独立危险因素(P均<0.05).结论 IMP3和IGF2蛋白的表达与胶质瘤的组织病理分级、肿瘤细胞增殖及患者临床预后密切相关,针对二者的靶点治疗有望成为胶质瘤的有效治疗方案.%Objective To investigate the expression of IMP3 and IGF2 and their influence on the prognosis of hu?man brain glioma. Methods Expression of IMP3, IGF2 and Ki-67 protein was examined using S-P immunohistochemsit?ry in 171 cases of gliomas and 20 cases of normal brain tissues. Kaplan-Meier and multivariate analysis of Variance were used to analysis the survival rate of patients with gliomas. Results Positive Expression levels of IMP3 ,IGF2 and Ki-67 in glioma tissue were significantly higher than those in normal brain tissue (both P<0.05). The expression levels of IMP3 , IGF2 and Ki-67 were positively correlated with the pathologic stage (P<0.05). Meanwhile, there was a significantly posi?tive correlation between IMP3 and IGF2 (rs=0.203, P<0.05). Further analysis of the results showed that both IMP3 and IGF2 positive expression levels were significantly associated with decreased overall survival (both P<0.05). Moreover, the expression of IMP3 and IGF2-positivity was a poor prognostic indicator for overall survival. Histological grading and expression of IMP3, IGF2 and Ki-67 were the independent risk factors of prognosis of gliomas (both P<0.05). Conclu-sion:IMP3 and IGF2 contribute to the progression of gliomas and influence the prognosis of patients with gliomas and the detection of IMP3 and IGF2 may be helpful to evaluate the course of disease and prognosis. They might be possible novel targets for molecular treatment of gliomas.