DNA聚合酶Ⅲ全酶的功能和结构的发现

摘要

介绍了DNA聚合酶Ⅲ和聚合酶Ⅲ+的发现，DNA聚合酶Ⅲ全酶形式的提出以及全酶亚基的分离。同时，介绍了DNA聚合酶Ⅲ全酶的结构和功能，其中包括α核心的聚合酶功能，ε亚基的3’→5’外切核酸酶活性．β亚基滑动夹子的功能以及Υ复合物的结构与功能。%The process of finding the structure and the function of DNA polymerase Ⅲ holoenzyme are introduced. The key events include that the discovery of DNA polymerase Ⅲ and Ⅲ * , the proposition of DNA polymerase Ⅲ holoenzyme, the separation of holoenzyme subunit, the function of core polymerase, the 3→5 exonuclease activity of ∈ subunit, the function of β subunit sliding clamp and γ complex.