种植脂肪干细胞的去细胞神经修复坐骨神经缺损的实验研究

摘要

目的 探讨脂肪干细胞(ADSCs)应用于组织工程化外周神经修复大鼠坐骨神经缺损的效果.方法 48只体重200～220 g的雌性F344大鼠随机分成6组,每组8只,分别用下面6种不同的实验组修复15 mm长坐骨神经缺损.A组:种植ADSCs的去细胞神经;B组:种植诱导ADSCs的去细胞神经;C组:种植许旺细胞(SCs)的去细胞神经;D组:去细胞神经;E组:自体神经移植;F组:空白对照.通过神经电生理检测、荧光金逆行示踪、组织学检测和坐骨神经功能指数测定评价各组修复神经缺损的效果.结果 术后12周,F组未见桥接物,A组和B组的神经电生理等各项指标均分别优于D组(P＜0.05或P＜0.01),与C组和E组间差异无统计学意义(P＞0.05).结论 初步结果显示ADSCs及诱导后ADSCs作为种子细胞,与去细胞神经构建的组织工程化外周神经移植体,能够修复外周神经缺损.%Objective To evaluate the effectiveness of using adipose-derived stromal cells (ADSCs)into a tissue-engineered peripheral nerve on bridging sciatic nerve gaps.Methods Forty-eight F344 female rats weighing 200 - 220 g were randomly divided into 6 groups of nerve grafting to repair 15 mm long asiatic nerve lesions,with 8 mrs in each group.Group A:ADSCs-laden acellular nerves;group B:differentiated ADSCs-laden acellular nerves;group C:Schwann cells-laden acellular nerves;group D:acellular nerves without cells;group E:autografts;group F:nonimplanted grafts.The effects were evaluated in terms of electrophysiulogy,Fluorogold retrograde tracing,histology and tracking studies.Results At 12 weeks after surgery,there was no graft bridging nerve gap in nonimplanted grafts.All the examinations of group A and B were better than group D,respectively (P＜0.05 or P＜0.01).But there were no statistically significant differences among group A,B,C,and D (P＞0.05).Conclusion ADSCs and differentiated ADSCs could promote nerve regeneration when used as seed cells to build tissue-engineered peripheral nerves with acellular nerve scaffolds.