HIV-1感染者慢性期治疗前后CD8+Tscm的特点研究

摘要

Objective To investigate the characteristics of CD8+ stem memory T cells (CD8+Tscm) in patients with chronic HIV-1 infection before and after antiretroviral therapy (ART) and to analyze their associations with progression of HIV-1 infection.Methods Thirty-six patients with chronic HIV-1 infection and 20 healthy subjects were enrolled in this study.Flow cytometry was performed to detect the percentages and absolute numbers of CD8+Tscm in patients with chronic HIV-1 infection before and after antiretroviral therapy (ART) as well as in healthy subjects.Correlation analysis was used to demonstrate the relationships between CD8+Tscm and markers for progression of HIV-1 infection (CD4+T cell count, HIV-1 viral load and level of activated T cells).Results The percentages and the absolute numbers of CD8+Tscm in patients with chronic HIV-1 infection had no significant change before and after ART.They were respectively positively correlated with the percentages and the absolute numbers of CD4+Tscm.The percentage of CD8+Tscm was proportional to the percentage of CD8+ central memory T cells (CD8+Tcm), but was inversely proportional to the percentage of CD8+ effector memory T cells (CD8+Tem).In addition, the percentages of CD8+Tscm in patients with HIV-1 infection were negatively correlated with the viral loads before ART.Conclusion CD8+Tscm are responsible for maintaining the homeostasis of other CD8+T cell subsets.CD8+Tscm play an important role in inhibiting viral replication.%目的 探讨HIV-1感染者慢性期治疗前后CD8+ Tscm(stem memory T cell)的特点及其与疾病进展的相关性.方法 选取36例HIV-1慢性期感染者和20名健康对照者.用流式细胞术检测健康对照者和HIV-1感染者抗病毒治疗前后CD8+Tscm的比例和数量.分析CD8+Tscm与其他CD8+T细胞亚群和疾病进展指标(CD4+T细胞数量、病毒载量和T细胞活化水平)的相关性.结果抗病毒治疗前后,HIV-1慢性期感染者CD8+Tscm的比例和数量均无显著性变化.HIV-1慢性期感染者CD8+Tscm的比例和数量均与CD4+Tscm的比例和数量呈正相关性.CD8+Tscm的比例与CD8+Tcm(central memory T cell)的比例成正比,与CD8+Tem(effector memory T cell)的比例成反比.此外,在抗病毒治疗前,HIV-1慢性期感染者CD8+Tscm的比例与病毒载量呈负相关.结论 CD8+Tscm参与维持其他CD8+T细胞亚群的稳态;CD8+Tscm参与抑制病毒的复制.