Baicalein Attenuates Severe Polymicrobial Sepsis via Alleviating Immune Dysfunction of T Lymphocytes and Inflammation

摘要

Objective:To investigate the effect of baicalein on polymicrobial sepsis-induced immune dysfunction and organ injury.Methods:A sepsis model was induced in Sprague-Dawley rats via caecal ligation and puncture(CLP).Specific pathogen free rats were randomly divided into a sham group,CLP group and CLP+baicalein(Bai)group(n=16 each).Rats in the CLP+Bai group were intravenously injected with baicalein(20 mg/kg)at 1 and10 h after CLP.Survival rate,bacterial load,and organ damage were assessed.Then each group was evaluated at 6,12,and 24 h to investigate the effect of baicalein on immune cells and inflammatory cytokines in septic rats.Results:Baicalein treatment significantly improved the survival of septic rats,decreased the bacterial burden,and moderated tissue damage(spleen,liver,and lung),as observed by haematoxylin and eosin staining.Septic rats treated with baicalein had strikingly increased proportions of CD3^(+)CD4^(+)T cells and ratios of CD4^(+)/CD8^(+)T cells in the peripheral blood and spleen(all P<0.05).Moreover,baicalein treatment decreased the apoptotic rate of whole white blood cells and spleen cells at 24 h after surgery(P<0.05).Baicalein significantly reduced the levels of tumor necrosis factor a and interleukin-6(IL-6)and increased IL-10,and the expression levels of galectin 9 were also raised in the spleen(P<0.01).Conclusion:Baicalein may be an effective immunomodulator that attenuates overwhelming inflammatory responses in severe abdominal sepsis.