基于高频交流电刺激的神经传导阻断的动物实验研究

摘要

Objective: To verify the blocking effect of high frequency signal to the nerve of the live animals. We used the sciatic nerve of live animals as the research object. We can obtain the best stimulation intensity, through observing the influence of electrical stimulation intensity and muscle contraction and adjust stimulation parameters continuously. Methods: The sciatic nerve was carefully exposed. We obtained the motor nerve conduction block in peripheral nerve by using high frequency electrical stimulation; then investigated the frequency-threshold relationship of the high frequency alternating current ( HFAC) blocking signal; and analyzed the influence of high frequency signals on the muscles which dominated by the corresponding nerves. Results: The results showed that the complete block could be achieved in a certain frequency and the different action of the muscle stiffness due to the different block frequency stimulation. Conclusions: The study provides more information about the conduction block induced by high frequency biphasic pulse current.%目的:面肌痉挛虽不危及生命,但可以对患者造成心理负担,严重影响患者正常的工作和生活.高频电刺激可以有效阻断运动神经的神经冲动,从而终止相应的神经功能.本研究通过利用动物在体神经进行实验的方法研究高频信号阻断神经传导的效应,为高频电刺激治疗面肌痉挛提供更多的信息.方法:采用Wistar大鼠在体坐骨神经进行实验,对神经施加高频阻断刺激信号,通过记录并观察神经所支配肌肉的肌电信号及其肌肉状态来确定阻断效果,研究高频交流电信号对周围神经传导阻断的作用.结果:当对神经给予高频阻断刺激信号时,神经出现传导阻断的现象,且阻断现象发生于某一频段,完全阻断则只是发生于其中更小的频率范围,而继续增大频率或幅值,则可能会出现部分阻断、强直甚至不阻断现象.结论:本动物实验研究为将来高频电刺激治疗面肌痉挛的临床应用的实现提供更多信息.