可吸收止血膜止血作用的实验研究

摘要

Bio-degradable hemostatic material has been widely applied during and after surgical operations.This study aimed to evaluate the safety and effectiveness of bio-degradable hemostatic material via a hepatorrhagia animal model of rabbit.Forty-five healthy New Zealand rabbits were divided into 3 groups.After the hepatorrhagia animal model of rabbit was established,2 groups were treated with bio-degradable hemostatic material and SURCHI-fibre,respectively,while the control group did not take any hemostatic treatment.Bleeding time and blood loss of each group were recorded during the operation.At 3,7 and 14 days after operation,B ultrasonic examination was performed for the detection of thrombus formation,and five rabbits were dissected for anatomical observation,thrombus observation,adhesion scoring and histological observation.Results show that bio-degradable hemostatic material can effectively shorten hemostatic time and reduce blood loss,compared with blank control group.In addition,bio-degradable hemostatic material was bio-degraded within 14 days,preventing the adhesions and thrombus formation,without obvious systemic toxicity.%可吸收止血材料目前在术中和术后创面渗血方面有着广泛的应用.通过研究可吸收止血膜在新西兰兔肝出血模型中的止血效果,评价可吸收止血膜的安全性和止血有效性.选择健康合格新西兰兔45只,随机分为3组.肝出血模型建立后,分别使用可吸收止血膜、壳聚糖基可吸收止血非织布(术益纱)进行止血,空白对照组不做止血处理.大体观察各组出血情况,记录出血时间及出血量.于术后第3、7和14天每个时间点用B超声波检查是否有血栓形成,每个时间点每组解剖5只家兔进行大体解剖检查、血栓观察、粘连观察评分、肝脏光镜下组织病理学检查.实验结果显示,与空白对照组比较,可吸收止血膜具有止血较快、减少出血量的作用,使用后第14天无肉眼可见残留,不产生组织粘连、血管血栓现象,无明显全身毒性反应.