Objective To develop a novel thermal treatment modality against metastatic tumor, and to verify the hypothesis that the extent of tumor angiogenesis damage and tumor cel necrosis, accompanied with immune suppression cel s relief is deterministic to enhance therapeutic effect in the thermal treatment. Methods The thermal treatment system was developed in our laboratory[1]. The treatment including hyperthermia and alternate treatment, were local y applied to 4T1 murine mammary carcinoma. The extent of tumor necrosis was examined. Further investigations were performed to study the changes of MDSCs in peripheral blood and spleen. Results The alternate treatment caused more damage to tumor microvasculature and tumor cell necrosis. Immunosuppression cells significantly reduced in peripheral blood and spleen. Moreover, it highly increased the survival rate of tumor-bearing mice. Conclusions The greatest destruction of primary tumor induced by the alternate treatment led to a relief of immune suppression in tumor bearing mice, and significantly increased therapeutic effect, especial y for metastatic tumor.% 目的发展新型冷热交替治疗转移性肿瘤，验证其对肿瘤血管和肿瘤组织损伤以及降低机体免疫抑制细胞是增强疗效的关键。方法基于自主研发的冷热交替治疗系统[1]，建立高转移性4T1乳腺癌模型经热疗或冷热交替治疗后观察肿瘤血管的损伤，肿瘤组织坏死程度，分析外周血中MDSCs的变化，脾脏中MDSCs浸润的改变。结果冷热交替治疗造成更严重的血管破坏和组织坏死，降低外周血和脾脏中MDSCs的含量，显著提高荷瘤小鼠的存活率。结论冷热交替治疗对原位肿瘤有较大杀伤作用，可以解除荷瘤小鼠全身的免疫抑制，显著提高转移性肿瘤疗效。
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