氧化钨(WO3-x@BSA)纳米颗粒的CT成像

摘要

Objective To investigate the value of CT taking WO3-x@BSA nanoparticles as contrast agents.Methods CT values of WO3-x@BSA nanoparticles and the iohexol contrast agents at different concentrations were measured.When the tube voltage was the same,CT values of the tungsten at different concentrations were measured,and the relationship curve of CT values and the concentrations,the tube voltages and CT values were plotted.The animal models were performed to observe CT imaging in vivo and in vitro.WO3-x@BSA nanoparticles were injected through tail vein and intraperitoneally of rats in vivo.In fresh pig liver,WO3-x@BSA nanoparticles were injected by portal vein.Results CT value of the tungsten was higher than the iodine at the same concentration,and the imaging signal of the tungsten was more significant than the iodine.The amount of WO3-x@BSA was less than the iohexol contrast agent reaching to the same CT value.WO3-x@BSA nanoparticles could reach the organs of various tissues in SD rats through different ways and showing the high density sig nal.WO3-x@BSA nanoparticles were also injected into the fresh in-vitro liver of pig,and could diffuse and distribute along with the blood vessels with good CT imaging.Conclusion As a novel CT contrast agent,WO3-x@BSA nanoparticles can be used to get good CT imaging.%目的 探讨氧化钨(WO3-x@BSA)纳米颗粒作为CT对比剂的CT成像价值.方法 测定以不同浓度WO3-x@BSA纳米颗粒、碘海醇为对比剂时增强CT的CT值;在管电压相同时,测定不同浓度钨的CT值;绘制浓度与CT值、电压与CT值的关系曲线.进行动物模型体内、外实验,通过尾静脉和腹腔2种注射方法将WO3-x@BSA纳米颗粒分别注入2只活体SD大鼠体内,经门静脉将WO3-x@BSA纳米颗粒注入新鲜离体猪肝,观察WO3-x@BSA纳米颗粒作为对比剂的CT成像效果.结果 相同浓度时,钨对比剂的CT值高于碘对比剂,图像密度更高;当WO3-x@BSA纳米颗粒对比剂浓度相同时,CT值随管电压的减小而增大.经不同途径注入大鼠体内的WO3x@BSA纳米颗粒均可到达各组织脏器,CT呈高密度.WO3-x@BSA纳米颗粒进入新鲜离体猪肝后沿血管进行扩散、浸润分布,CT成像效果良好.结论 WO3-x@BSA纳米颗粒作为对比剂CT成像效果良好.