几种氮氧自由基在植物中rn稳定性的ESR研究

摘要

为了在植物中合理地应用自旋标记物，文中比较了几种氮氧自由基在植物体中的稳定性.在相同的条件下，将这几种氮氧自由基的稀溶液通过渗吸的方法导入植物体.在不同时间、植物体不同部位，用电子顺磁共振（ESR）方法检测信号的强弱程度，并且观察植株在吸入含氮氧自由基的稀释液后，生长状况的变化.发现能够在植物体中稳定存在、信号强、对植株毒害小的氮氧化合物的几个特点.如；分子量较小,大多为五元环的吡咯衍生物.绘制了氮氧自由基在植株中随时间而降解的曲线，通过比较发现，以浓度为10-4mol/L的稀释液浸泡，可以得到较好的顺磁信号，同时也不影响植物的生长.这一研究将对植物整体植株的顺磁成像技术的发展有帮助，同时有可能成为一种植物生理检测的方法.%In order to reasonably use nitroxides in plant, We compared the stability of several nitroxide compounds in plant. Under the same condition, the compound was lead into plant though soaking in the diluted solution. At different time, the ESR spectra of different parts of plant were measured and the growth status of plant were observed. The characters of spin probes, which can exist steadily in plant with high signal and less toxicity are found. Usually they are derivatives of pyrrolidine with small molecular weight. From the kinetic curves and growth states of the plant, it was found that the concentration of 10-4mol/L of spin probe was proper for using. The results are helpful for the development of plant ESR imaging and may be developed to a new method for measuring plant physiology.