常规T2像和组织学研究APPswe/PS1dE9转基因小鼠模型

摘要

用常规的T2加权像研究阿尔茨海默病双转基因APPswe/PS1dE9小鼠模型,拟合了海马和顶叶皮层的T2,并用组织学方法研究了APP/PS1小鼠在这2个感兴趣区域随年龄相关的淀粉样斑块病理的发展及铁质的沉积.T2拟合的结果显示,较高月龄的APP/PS1小鼠相比于低月龄APP/PS1小鼠T2有升高但不显著,各月龄组APP/PS1小鼠与同窝生非阳性小鼠之间也无明显差异;组织学的结果则显示年龄相关的淀粉样斑块的增多,铁质在16月龄的APP/PS1小鼠脑区中有明显沉积且和斑块具有"共域性".两方面的实验结果提示:在该模型淀粉样斑块沉积病理发展的早期,这2个脑区的T2并不能作为特异性的病理发展的标志.%We studied a double transgenic APPSwe/PSldE9 mouse model using T2weighted imaging and histological assessment to detect age-related amyloidosis and iron load in the hippocampus and cortex. The results indicated that T2 of older transgenic mice was slightly higher than that of the younger ones, but the difference did not reach statistical significance. We also did not found any significant T2 difference between transgenic mice and age-matched non-transgenic littermates. Histological assessment revealed an age-related increase of amyloid load in the hippocampus and parietal cortex.Positive iron staining was observed in amyloid-rich regions of 16-month-old APP/PS1 mice, and co-localized, to a large extent, with amyloid deposits. These results indicated that T2 might not be sensitive enough to detect amyloid and iorn deposition in this model.