循环microRNA检测技术及分析前质量控制

摘要

Circulating microRNAs (miRNAs) have been confirmed to play an important role in biological processes,such as cell proliferation,differentiation and apoptosis.Founded on the high stability and minimal invasiveness,circulating miRNAs could be applied to the clinical practice as biomarkers.There are many technological approaches and platforms for circulating miRNAs detection but without a consistent protocol.Meanwhile,there are various factors affecting circulating miRNAs detection.In this review,technical factors and preanalytical factors in the circulating miRNA profiling are explored.%miRNA在许多疾病中起着重要作用,如与细胞增殖、分化和凋亡等相关.鉴于循环miRNA的稳定性,血液标本获取的无创性,因此它可作为生物标志物应用于临床.目前miRNA检测方法多样,影响其检测的因素很多,无统一操作规程,本文即对miRNA的检测技术及分析前影响因素进行探讨.